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Efficiently Isolate Liquid Biopsy CTCs to Reveal the Secrets of Metastasis
CTCs offer substantial utility for studying, staging, and monitoring cancer in liquid biopsy samples. The molecular and genomic characterization of CTCs can contribute to improving disease diagnosis and personalizing treatment, but they are rare and can be difficult to collect.
Fast, efficient, and sensitive isolation of circulating tumor cells from liquid biopsy samples is essential for successful insights into the mechanisms of metastasis. Initial CTC enumeration, immunolabeling, and culturing, as well as downstream digital PCR (dPCR), sequencing and gene expression analyses are dependent upon effective isolation that preserves the native biology of each tumor cell.
Celselect Slide™ Technology
The Next Innovation in CTC Detection
Bio-Rad's new Genesis Cell Isolation System with Celselect Slide Technology is an innovation in automated CTC detection that uses microfluidic physical size-selection to gently capture CTCs and rare cells in <1 hour for downstream applications.
Benefits of Celselect Slide Technology
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Capture mechanism
Viable cell isolation based on size, removal of >99% of red blood cells (RBCs) and white blood cells (WBCs)
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Two applications
On-slide enrichment and enumeration
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Sample processing
High capture efficiency without pre-processing
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Customization
Optimize protocols and employ user-defined antibodies
Celselect Workflows — One Platform, Two Applications
Enrichment Workflow
Liquid biopsy sample is prepared with dilution buffer and added to the sample inlet. CTCs are isolated automatically in the Celselect Slide while RBCs and WBCs pass through. Cells are retrieved in a single tube for downstream analyses.
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1. Prepare Liquid Biopsy Sample
Add buffer to liquid biopsy sample and add to sample inlet.
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2. Isolate and Retrieve Cells
Genesis System automates isolation and retrieval of cells.
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3. Downstream Analyses
Cells can be characterized with methods such as sequencing (bulk or single-cell), digital PCR, FISH, Flow Cytometry or cells cultured for further in vitro and in vivo analysis.
Enumeration Workflow
Liquid biopsy sample is prepared with fixing reagent and added to the sample inlet. CTCs are isolated automatically in the Celselect Slide while RBCs and WBCs pass through. The Celselect Slide can be viewed with a fluorescent microscope in the lab. Image files can then be analyzed.
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1. Prepare Liquid Biopsy Sample
Add fixing reagent to liquid biopsy sample and add to sample inlet. Sample and staining reagents are prepped according to predefined protocols.
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2. Isolate Cells, Immunostaining
Genesis System automates isolation and immunostaining on Celselect Slides.
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3. Image
Celselect Slide are imaged with a fluorescent microscope in the lab.
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4. Create Report
Import image files from microscope for analysis, choose cells to include and generate report.
Application and Uses
Celselect Slide Enrichment
- Capture and retrieval of CTCs from liquid biopsies
- Downstream analysis such as cell culture, FISH, NGS or qPCR/ddPCR and more…
Celselect Slide Enumeration
- Highly efficient capture of CTCs from liquid biopsies
- On-slide immunostaining
- Imaging on compatible Automated Microscopes
- Image Viewer Software for result reporting
Resources
Celselect Slide Technology Application Note
Discover the advantages of Celselect Technology in CTC and ctDNA analyses.
Celselect Publication List
See Celselect Slides in action for translation oncology, diagnostics, and more.
Related Products
Genesis System
Visit the Genesis System product page to view full system details.
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Celselect Slide Enumeration Direct Stain Kit
For CTC capture and staining via labeled-primary antibody.
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Celselect Slide Enumeration Indirect Stain Kit
For CTC capture and staining via labeled-secondary antibody.
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Celselect Slide Enrichment Kit
For CTC retrieval and downstream analysis (sequencing, dPCR, FISH, western blotting, etc).
