Circulating Tumor Cell (CTC) Enrichment and Enumeration

CTC and rare cell selection

Efficiently Isolate Liquid Biopsy CTCs to Reveal the Secrets of Metastasis

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CTCs offer substantial utility for studying, staging, and monitoring cancer in liquid biopsy samples. The molecular and genomic characterization of CTCs can contribute to improving disease diagnosis and personalizing treatment, but they are rare and can be difficult to collect.

Fast, efficient, and sensitive isolation of circulating tumor cells from liquid biopsy samples is essential for successful insights into the mechanisms of metastasis. Initial CTC enumeration, immunolabeling, and culturing, as well as downstream digital PCR (dPCR), sequencing and gene expression analyses are dependent upon effective isolation that preserves the native biology of each tumor cell.

Celselect Slide™ Technology

The Next Innovation in CTC Detection

Bio-Rad's new Genesis Cell Isolation System with Celselect Slide Technology is an innovation in automated CTC detection that uses microfluidic physical size-selection to gently capture CTCs and rare cells in <1 hour for downstream applications.

 

Benefits of Celselect Slide Technology

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    Capture mechanism

    Viable cell isolation based on size, removal of >99% of red blood cells (RBCs) and white blood cells (WBCs)

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    Two applications

    On-slide enrichment and enumeration

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    Sample processing

    High capture efficiency without pre-processing

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    Customization

    Optimize protocols and employ user-defined antibodies

Celselect Workflows — One Platform, Two Applications

Enrichment Workflow

Liquid biopsy sample is prepared with dilution buffer and added to the sample inlet. CTCs are isolated automatically in the Celselect Slide while RBCs and WBCs pass through. Cells are retrieved in a single tube for downstream analyses.

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    1. Prepare Liquid Biopsy Sample

    Add buffer to liquid biopsy sample and add to sample inlet.

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    2. Isolate and Retrieve Cells

    Genesis System automates isolation and retrieval of cells.

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    3. Downstream Analyses

    Cells can be characterized with methods such as sequencing (bulk or single-cell), digital PCR, FISH, Flow Cytometry or cells cultured for further in vitro and in vivo analysis.

Enumeration Workflow

Liquid biopsy sample is prepared with fixing reagent and added to the sample inlet. CTCs are isolated automatically in the Celselect Slide while RBCs and WBCs pass through. The Celselect Slide can be viewed with a fluorescent microscope in the lab. Image files can then be analyzed.

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    1. Prepare Liquid Biopsy Sample

    Add fixing reagent to liquid biopsy sample and add to sample inlet. Sample and staining reagents are prepped according to predefined protocols.

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    2. Isolate Cells, Immunostaining

    Genesis System automates isolation and immunostaining on Celselect Slides.

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    3. Image

    Celselect Slide are imaged with a fluorescent microscope in the lab.

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    4. Create Report

    Import image files from microscope for analysis, choose cells to include and generate report.

Application and Uses

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    Celselect Slide Enrichment

    • Capture and retrieval of CTCs from liquid biopsies
    • Downstream analysis such as cell culture, FISH, NGS or qPCR/ddPCR and more…
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    Celselect Slide Enumeration

    • Highly efficient capture of CTCs from liquid biopsies
    • On-slide immunostaining
    • Imaging on compatible Automated Microscopes
    • Image Viewer Software for result reporting

Resources

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