As one-of-a-kind flow cytometer with well-rounded versatility and capabilities, the ZE5 Cell Analyzer has helped scientists in both academia and industry accelerate scientific and drug discoveries. Read more to learn how.
Cell Analysis System

ZE5 Cell Analyzer
The ZE5 Cell Analyzer is a high performance, automation-ready flow cytometer with the ideal combination of high-throughput screening and high parameter cell analysis capabilities.
- Integrated sample loader supporting tube racks and multiple plate formats without hardware exchange
- Up to 5 lasers, 27 colors enabling panel design flexibility
- Acquisition at up to 100,000 events per second without data abort
- Process 384-well plates in less than 50 min and 96-well plates in less than 15 min in high-throughput mode
- Sensitive small particle detector for exosome analysis
- Device-agnostic Application Programming Interface (API) for integration with any laboratory robotic system
- Future proofing with a variety of optical and fluidic upgrade options
Product Details | Brochure | System Tour
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FCS Express
Streamline your acquisition-to-publication workflow with the FCS Express Software from De Novo that comes with new ZE5 Cell Analyzer purchases.
ZE5 Configurations for FRET and Fluorescent Protein Assays
Learn about new, high-throughput capabilities of the ZE5 Cell Analyzer that help overcome limitations of fluorescence microscopy during the analysis of protein interaction and protein assembly in living cells.
Flow Cytometry Antibodies
Select from over 11,000 primary and secondary antibodies and related reagents for flow cytometry, immunology, cancer research, veterinary, and other applications.
Automated High-Throughput Screening
Therapeutics development, antibody screening and biomarker discovery demand maximal productivity to reduce time-to-results, which can greatly benefit from high-throughput, high parametric flow analysis interfaced with robotic automation.
With its high-throughput-ready hardware and software, agnostic API, and external fluidics upgrade options, the ZE5 Cell Analyzer is the long-awaited solution for scientists’ high productivity need with added benefit of superior performance and flexibility.
Speed up your Discovery without Compromise
100,000 events per second without data loss.
96-well plates in < 15 min
384-well plates in < 50 min
ensuring low carryover at high speed with high throughput screeningUnattended operation with external fluidics and robotic automation
SAVE TIME
with flexible import options for routine experiments

ZE5 Cell Analyzer in High-Throughput Screening
Publications

Considerations for Automating your ZE5 Cell Analyzer
(PDF 0.46 MB)
See what should be considered and what can be accomplished when integrating you flow cytometer into a laboratory robotic system
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Automated ZE5 Cell Analyzer in Action
Automated high-throughput screening is enabled with the ZE5 Cell Analyzer integrated into any laboratory robotic system such as Biosero’s Green Button Go
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Get Reliable Results Faster
Discover why the ZE5 Cell Analyzer is a better solution for your high throughput screening needs
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From Acquisition to Analysis
Learn how to analyze your plate-based high throughput screening data with FCS Express bundled with the ZE5 Cell Analyzer

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More Applications
ZE5 Cell Analyzer is designed to meet a broad range of application needs. Check out these examples to learn how the ZE5 Cell Analyzer has helped scientists obtain more high quality results in less time from a variety of research areas
Cell-Mediated Immunity to SARS-CoV-2
Potent SARS-CoV-2-specific T cell immunity is documented in COVID-19 convalescent patients, along with prevalent cross-reactivity between circulating “common cold” coronaviruses and the novel coronavirus, SARS-CoV-2.
CAR-T Cell Characterization
Utilizing CRISPR-mediated genetically modified CAR-T cells to treat T cell malignancies without CAR-T fratricide.
Extracellular Vesicle Labeling and Uptake Analysis
Check out how the ZE5 Cell Analyzer was used to characterize parasite-derived extracellular vesicles (EVs) that are smaller than 200 nm.
Immune Profiling during Stem Cell Transplantation
(PDF 0.38 MB)A 21-color panel developed on the ZE5 Cell Analyzer enables researchers to monitor immune profiles before and after stem cell transplantation, and to predict treatment outcomes.
Microbial Analysis
This research Identifies an eukaryotic antiviral pathway that has ancient evolutionary roots stemming from protection of bacteria against phage infection.
Hematopoietic Stem Cell Phenotyping
Kinetics of HSC differentiation is characterized with high-dimensional single-cell analyses on the ZE5 Cell Analyzer and with scRNA-Seq
Monitoring T Cell Exhaustion After Immunotherapy
(PDF 0.77 MB)Read how the multi-parametric capability of the ZE5 Cell Analyzer enables monitoring of T cell exhaustion after Nivolumab Treatment
Exosome Analysis
(PDF 0.52 MB)Read how to readily detect and analyze exosomes on the ZE5 Cell Analyzer with various detection methods
Resources
ZE5 Cell Analyzer Publication List
(PDF 0.58 MB)A fast-growing list of ZE5 publications
Flow Cytometry Basics Guide
A basic overview of all the important facets of flow cytometry
Fluorescent Spectraviewer
A unique, intuitive fluorescent spectraviewer customized for your applications of interest
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