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Using ddPCR™ Technology for Rapid and Accurate Detection of Cell Line Edits
Optimizing cell lines using gene editing techniques is a critical aspect in antibody discovery and development. Advances in editing technology, such as the advent of the CRISPR-Cas9 system, have expanded the diversity of cell lines — and consequently, the therapeutics that can be produced. Confirming these edits early during clonal expansion is crucial to avoid lost time and resources on expanding clones that do not contain desired edits. Until now, scientists have been limited to sequencing and gel-based detection methods, which are slow and can lack precision. Droplet Digital™ PCR (ddPCR™) is a rapid and sensitive technique that can enable faster, more accurate confirmation of cell line edits.
Read this article to learn:
- How ddPCR technology can detect homology-directed repair events with a sensitivity of 0.02%
- How ddPCR technology can be used to detect single base pair indels at frequencies as low as 0.3%
- How ddPCR technology can determine the relative contribution of different gRNAs to overall Cas9 nickase activity
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