2-D SDS-PAGE Standards #1610320


500 μl, 17,500–76,000 MW range, pI 4.5–8.5, unstained mixture of myoglobin, trypsin inhibitor, CA, GAPDH, actin, BSA, and conalbumin

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Number Description Options
161-0320-MSDS 2-D SDS-PAGE Standards English

Use 2-D SDS-PAGE Standards for spot matching and molecular weight determination of proteins on 2-D PAGE gels. Our 2-D SDS-PAGE Standards are a mixture of seven proteins (17.5–76 kD, with pI 4.5–8.5). The seven reduced, denatured proteins can be visualized with silver or Coomassie Blue stains. No dilution is required.

Applications and Uses

  • PI and MW determination of sample proteins
  • 2-D gel matching
  • An internal control to assess reproducibility

2-D SDS-PAGE Standards Specifications

Size Range Quantity Recommended Loading Volume
7 spots, 17.5–76.0 kD,
with pI 4.5–8.5
~0.30 mg protein in 500 µl of 9 M urea, 5% 2-mercaptoethanol, 2% Bio-Lyte® 5/7 ampholyte Mini gels: 2.5 µl (Coomassie R-250 stain); 0.5–2.5 µl (silver stain)

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