Total Protein Blot Stains

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Total Protein Blot Stains

Overview

Specifications
Products Sensitivity Staining Time Storage Condition Shelf Life
Ruby Blot Stain 2–8 ng <1 hr Ambient 6 months
Coomassie Blue R-250 100–1,000 ng 1 hr Ambient 1 year
Colloidal Gold Total Protein Stain 1 ng 2 hr 4°C 6 months
Total Protein Stain Comparison
Comparison of Total Protein Staining Methods
Stain Sensitivity Staining Time Advantages Disadvantages
SYPRO Ruby protein blot stain 2–8 ng <1 hr Mass spectrometry compatible UV fluorescence detection system required
Coomassie Blue R-250 100–1,000 ng ~1 hr Inexpensive, rapid stain Low sensitivity, shrinks nitrocellulose membranes
Colloidal gold total protein stain 1 ng ~2 hr Very sensitive, rapid stain High background with nylon membranes
SYPRO Ruby Spectra

Fluorescence excitation and emission spectra of SYPRO Ruby protein gel stain.

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Detection of all proteins on western blots provides a visual image of the electrophoretic pattern, which helps identify specific antigens in a complex protein mixture. Western blot stains have a sensitivity comparable to gel stains.

Bio-Rad offers three staining options for total protein detection:

SYPRO Ruby protein blot stain — provides highly sensitive detection of proteins on PVDF or nitrocellulose membranes. The stain makes it possible to visualize sample proteins and molecular weight standards directly on the blot, eliminating the need to stain duplicate gels or guess at protein transfer efficiencies. After staining, target proteins can be detected by colorimetric or chemiluminescent immunostaining or analyzed by microsequencing or mass spectrometry with no interference from the stain.

Coomassie Brilliant Blue R–250 dye — anionic dye used for staining gels and PVDF and nylon membranes. It is a rapid and inexpensive stain that can detect nanogram levels of protein. Since this dye can interfere with antibody binding sites, subsequent immunodetection of proteins is not recommended.

Colloidal gold total protein stain — stabilized gold solution optimized for rapid and sensitive identification of proteins bound to nitrocellulose membranes (Rohringer and Holden 1985). Protein bands stain dark red following incubation of the membrane with colloidal gold solution. The stained membrane yields a permanent record of the protein pattern for exact comparison to immunostained results. Colloidal gold total protein stain is provided ready to use.

Reference
Rohringer R and Holden DW (1985). Protein blotting: detection of proteins with colloidal gold, and of glycoproteins and lectins with biotin-conjugated and enzyme probes. Anal Biochem 144, 118–127.

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SYPRO Ruby Protein Blot Stain

1703127
200 ml, total protein stain for use on PVDF or nitrocellulose membrane

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Coomassie Brilliant Blue R-250

1610400
10 g, Coomassie Brilliant Blue R-250 protein stain powder

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Colloidal Gold Total Protein Stain

1706527
500 ml, ready-to-use stain for rapid and sensitive protein identification on nitrocellulose or PVDF membranes

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Number Description Options
2032 Western Blotting Detection Reagents Brochure, Rev F Click to download
LIT294 Instruction Manual, Colloidal Gold Total Protein Stain, Rev D Click to download
4006173 Instruction Manual, SYPRO Ruby Protein Stains, Rev B Click to download
6148 Transfer of High Molecular Weight Proteins to Membranes: A Comparison of Transfer Efficiency Between Blotting Systems, Rev B Click to download
6493 Bio-Rad’s Host Cell Protein (HCP) Workflow Quick Guide Click to download [ Add to Cart (Free) ]

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