Reactive Surfaces

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Overview

ProteinChip Array Selection Guide
General Description Array Surface Chemistry (active spots) Applications
Strong Anion Exchange
Capture of molecules that have negative surface charges Q10 Cationic, quaternary ammonium groups that interact with the negative charges on the surface of target proteins; for example, aspartic or glutamic acid
  • Selective analysis of proteins with low pls
  • Biomarker discovery
Weak Cation Exchange
Capture of molecules that have positive surface charges CM10 Weak anionic carboxylate groups that interact with the positive charges on the surface of the analyte; for example, lysine, arginine, or histidine
  • Selective analysis of proteins with high pls
  • Biomarker discovery
IMAC
Capture of molecules that bind polyvalent metal ions, such as nickel, copper, zinc, iron, and gallium IMAC30 NTA groups that chelate metal ions. Proteins applied to the array surface may bind to the chelated metal ion through histidine, tryptophan, cysteine, and phosphorylated amino acid residues
  • Analysis of metal-binding, phosphorylated, or polyhistidine (His)-tagged proteins
  • Biomarker discovery
Hydrophobic/Reverse-Phase
Capture of large proteins through hydrophobic or reverse-phase interactions H50 Methylene chains that closely mimic the characteristics of C6 to C12 alkyl chromatography media
  • Protein profiling
  • Purity determination
  • Rapid protein analysis
  • Calibration with proteins of known MW
  • Biomarker discovery
Capture of smaller proteins and peptides through hydrophobic and reverse-phase interactions H4* Active spots contain chains of 16 methylene groups that bind proteins through reverse-phase chemistry. Binds proteins abundant in alanine, valine, leucine, isoleucine, phenylalanine, tryptophan, or tyrosine
  • Purity determination
  • Rapid protein/peptide analysis
  • Biomarker discovery
Analysis of peptides resulting from tryptic digestion of proteins SEND ID Chains of 18 methylene groups (C18) that bind peptides through reverse-phase chemistry; matrix is integrated into the array surface
  • Peptide mass fingerprinting
Normal Phase
General protein binding surface; recommended for hydrophilic proteins NP20 Silicon dioxide, which allows proteins to bind via serine, threonine, or lysine residues
  • Quick protein analysis
  • Purity determination
  • Verification of the presence or absence of a molecule
  • Calibration with peptides or proteins of known MW
Preactivated Surface
Covalent immobilization of biomolecules for subsequent specific capture of proteins from complex samples PS10 Reactive acyl imidazole moieties
  • Analysis of antibody-antigen, receptor-ligand, protein-protein interactions
  PS20 Epoxy groups  
  RS100 Reactive acyl imidazole moieties on a hydrogel backbone  
Nonreactive Surface
  Au Gold provides a nonreactive surface for general protein analysis (limited reusability)
  • Quick protein analysis
  • Calibration with peptides or proteins of known MW

*Array lacks the hydrophobic coating needed for sample containment; a hydrophobic PAP pen can be used to manually create the barrier.

SELDI Citations
SELDI Citations Database
 

SELDI Citations Database

ProteinChip reactive surface arrays feature chemically modified surfaces that form covalent bonds with biologically relevant molecules such as antibodies or receptors. These arrays are designed for use in immunoassays and receptor-ligand binding studies. Arrays feature eight 2 mm diameter spots (A–H format) that correlate with the spacing of wells in a single column on a standard 96-well microplate (9 mm well pitch). A unique bar code and serial number are printed on the back of each array for traceability.

Refer to the ProteinChip Array Selection Guide for more information on selecting arrays with the appropriate surface chemistries.

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(Discontinued) ProteinChip Gold Array

C5530033
Pkg of 1, A–H format, gold array for use with the ProteinChip SELDI system

This product is no longer available. Please contact technical support for more information on replacement product.
ProteinChip PS10 Arrays

C5530044
Pkg of 12, A–H format, preactivated (reactive acyl imidazole moieties) arrays for use with the ProteinChip SELDI system

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$1,155.00
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ProteinChip PS20 Arrays

C5730045
Pkg of 12, A–H format, preactivated (epoxy surface groups) arrays for use with the ProteinChip SELDI system

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$987.00
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ProteinChip RS100 Arrays

C5530082
Pkg of 6, A–H format, preactivated (reactive acyl imidazole moieties) arrays for use with the ProteinChip SELDI system

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$987.00
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Number Description Options
5530 ProteinChip SELDI System Brochure, Rev A Click to download
5524 ProteinChip Arrays and Reagents Product Information Sheet, Rev C Click to download
10008253 Instruction Manual, ProteinChip PS10 Array (Preactivated Surface), Rev D Click to download
10008240 Instruction Manual, ProteinChip RS100 Array (Reactive Surface), Rev C Click to download
10008250 Instruction Manual, ProteinChip Cassette-Compatible Bioprocessor, Rev D Click to download
10008234 Instruction Manual, ProteinChip PS20 Array (Preactivated Surface), Rev F Click to download