ProteinChip Buffers

Print

Overview

SELDI Citations
SELDI Citations Database
 

SELDI Citations Database

Request a Biomarker Discovery Guide

Biomarker Discovery Using SELDI Technology

Proper study and experimental design coupled with the correct data analysis approach are critical components of successful biomarker discovery, no matter which technology, platform, or workflow is used. Bio-Rad has produced two in-depth guides to provide researchers with strategies for success.

The first guide, Biomarker Discovery Using SELDI Technology — A Guide to Successful Study and Experimental Design, provides a series of general guidelines for effective study design using virtually any proteomics technology, and gives specific recommendations for using the ProteinChip surface-enhanced laser desorption/ionization (SELDI) system. This guide also includes:

  • Guidelines for proper sample selection, collection, handling, and storage
  • Instructions for planning the experimental workflow
  • Tips on improving reproducibility through standard operating procedures
  • Important considerations for data analysis implementation
  • Optimized methods and protocols for serum or plasma analysis


The second guide, Biomarker Discovery Using SELDI Technology — A Guide to Data Processing and Analysis Using ProteinChip Data Manager Software, is a comprehensive resource for data analysis workflows, guidelines, and specific recommendations for analyzing biomarker discovery data. The guide covers:

  • General data analysis workflows
  • Data organization
  • Data processing steps, including baseline subtraction, normalization, and spectrum alignment
  • Statistical analysis using P values and ROC curves, hierarchical clustering, and principal component analysis
  • Glossary of software terms

Premade ProteinChip buffers are optimized for use with a variety of ProteinChip arrays. These buffers and buffer sets are pH-tested to ensure they are within 0.05 of the specified pH for reproducible results. The addition of an antimicrobial preservative ensures reliable performance.

(Discontinued) ProteinChip CM High-Stringency Buffer

K20-00004
200 ml, ProteinChip CM high-stringency buffer, for use with ProteinChip CM10 arrays (#C57-30075)

This product is no longer available. Please contact technical support for more information on replacement product.
(Discontinued) ProteinChip H50 Buffer

K20-00001
200 ml, 10% acetonitrile, 0.1% trifluoroacetic acid, and antimicrobial preservatives, for use with ProteinChip H50 arrays (#C57-30065)

This product is no longer available. Please contact technical support for more information on replacement product.
Hazardous  
(Discontinued) ProteinChip H50 Buffer

K20-00005
1 L, 10% acetonitrile, 0.1% trifluoroacetic acid, and antimicrobial preservatives, for use with ProteinChip H50 arrays (#C57-30065)

This product is no longer available. Please contact technical support for more information on replacement product.
Hazardous  
(Discontinued) ProteinChip IMAC Binding Buffer

K20-00006
1 L, 0.1 M sodium phosphate, 0.5 M sodium chloride, pH 7, and antimicrobial preservatives, for use with ProteinChip IMAC arrays (#C57-30078)

This product is no longer available. Please contact technical support for more information on replacement product.
(Discontinued) ProteinChip IMAC Charging Solution

K20-00008
200 ml, 0.1 M cupric sulfate solution and antimicrobial preservatives, for use with ProteinChip IMAC arrays (#C57-30078)

This product is no longer available. Please contact technical support for more information on replacement product.
(Discontinued) ProteinChip IMAC Neutralizing Solution

K20-00009
200 ml, 0.1 M sodium acetate, pH 4, and antimicrobial preservatives, for use with ProteinChip IMAC arrays (#C57-30078)

This product is no longer available. Please contact technical support for more information on replacement product.
Number Description Options
5530 ProteinChip SELDI System Brochure, Rev A Click to download
5524 ProteinChip Arrays and Reagents Product Information Sheet, Rev C Click to download
10008247 Instruction Manual, ProteinChip CM High-Stringency Buffer, Rev G Click to download
10008241 Instruction Manual, ProteinChip IMAC Buffer Set, Rev E Click to download
10008242 Instruction Manual, ProteinChip CM Low-Stringency Buffer, Rev E Click to download
10008258 Instruction Manual, ProteinChip H50 Buffer, Rev F Click to download