Prepacked Gravity Flow Columns for Ion Exchange

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Overview


Specifications
Catalog # Description Particle Size (µm) Ionic Form Application
731-6211 Poly-Prep Columns AG1-X8 resin, 100–200 mesh, 50 106–180 Chloride Separation of low molecular weight inorganic ions
731-6212 Poly-Prep Columns AG1-X8 resin, 200–400 mesh, 50 45–106 Chloride For high-resolution random separations
731-6221 Poly-Prep Columns AG1-X8 resin, 200–400 mesh, 50 45–106 Formate Separation of low molecular weight biological compounds such as nucleotides, peptides and caarboxylic acids
731-6213 Poly-Prep Columns AG50W-X8 resin, 100–200 mesh, 50 106–250 Hydrogen Separation and concentration of low molecular weight cations such as small peptides and amino acids
731-6214 Poly-Prep Columns AG50W-X8 resin, 200–400 mesh, 50 63–150 Hydrogen For high-resolution random separations
731-1555 Poly-Prep Column Stack Cap. 50     Allows connection of pumps, reservoirs, or columns in series
Prepacked Cartridge Selection by Application
Cartridge by Application Support Type of Separation Chemical Form Protein capacity/ml*
Protein and Plasmid Purification
High Q Macro-Prep® High Q Strong anion exchange -N+(CH3)3 ≥37 mg BSA
UNOsphere Q UNOsphere™ Q Strong anion exchange -N+(CH3)3 180 mg BSA
UNOsphere S UNOsphere S Strong cation exchange -SO3- 60 mg human IgG
DEAE Macro-Prep DEAE Weak anion exchange -HN+(C2H5)2 >35 mg protein
Protein Purification
High S Macro-Prep High S Strong cation exchange -SO3- ≥49 mg human IgG
IgG Purification
DEAE Blue DEAE Affi-Gel® Blue Affinity Cibacron Blue
F3GA and DEAE
0.2–1.0 ml serum
Antibody Purification
Protein A Affi-Prep® protein A Affinity Protein A 8–10 mg mouse monoclonal IgG1
CHT™ Type I CHT ceramic
hydroxyapatite Type I
Hydroxyapatite (Ca5(PO4)3OH)2 16–23 mg human IgG
CHT Type II CHT ceramic
hydroxyapatite Type II
Hydroxyapatite (Ca5(PO4)3OH)2 25–60 mg human IgG
Affinity-Tagged Protein Purification
IMAC** Profinity™ IMAC Affinity Iminodiacetic acid ≥15 mg recombinant
His-tagged protein
GST** Profinity GST Affinity Glutathione ≥10 mg/ml recombinant
GST-tagged protein
Desalting, Buffer Exchange
P-6 Bio-Gel® P-6 Gel filtration Polyacrylamide 10–22% CV***
Purification of Serum Proteins, Enzymes
Blue Affi-Gel Blue Affinity Cibacron Blue
F3GA
0.2 mlm serum
11 mg albumin

*   Protein capacity based on bulk support; check individual instruction manuals for run conditions and specifications.
**  IMAC and GST cartridges are designed for use with the Profinia protein purification system.
*** Column volume.

Chemical Compatibility
Chemical Compatibility of Column
Chemical Compatibility*
Acetic acid, 50% S
Aceton S
Acetonitrile S
Ammonium hydroxide S
Chloracetic acid S
Chromic acid, 50% U
Diethyl ether L
Dimethyl formamide S
Dimethylsulfoxide S
Ethyl alcohol S
Ethylene glycol S
Formamide S
Glacial acetic acid S
Glycerine S
Hydrochloric acid, 35% S
Isobutyl alcohol S
Isopropyl alcohol S
Methanol S
Perchloric acid U
Phosphoric acid S
Propylene glycol S
Sodium hydroxide, 50% to Sat. L (U for anions)
Sulfuric acid, 60% S (U for anions)
Sulfuric acid, 98% U
Urea, 8M S

* S = Satisfactory U = Unsatisfactory L = Low

Ion Exchange Capacity
Resin Ion Exchange Capacity
(nominal meq/2 ml of resin)
 
AG® 1-X8 resin 2.4
AG 2-X8 resin 2.4
AG 50W-X4 resin 2.2
AG 50W-X8 resin 3.4
Chelex® 100 resin 0.8
Bio-Rex® 70 resin 4.8
Working Temperature
Resin Maximum Temperature (°C)
AG® 50 resin 150
AG 1 resin 150 (50°C if converted to OH- form)
AG 2 resin 150 (30°C if converted to OH- form)
Chelex® 100 resin 75
Bio-Rex® 70 resin 100



Poly-Prep® prepacked columns for gravity-flow chromatography provide convenience for sample preparation and other small-scale applications. The graduated 0.8 x 4 cm columns are constructed of polypropylene. Each column holds a standard bed volume of 2 ml AG® ion exchange resin and includes an integral 10 ml reservoir. A Poly-Prep stack cap, which allows connection to pumps, reservoirs, or columns in series, is also available.

 

Poly-Prep® Columns, AG® 1-X8, chloride form

731-6211
Pkg of 50, 9 cm high, 2 ml of prepacked analytical grade 1-X8 resin, 100–200 mesh, 106–180 particle size, chloride form, 10 ml reservoir

List Price:   $225.00
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Poly-Prep® Columns, AG® 1-X8, chloride form

731-6212
Pkg of 50, 9 cm high, 2 ml of prepacked analytical grade 1-X8 resin, 200–400 mesh, 45–106 particle size, chloride form, includes 10 ml reservoir

List Price:   $225.00
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Poly-Prep® Columns, AG® 1-X8, formate form

731-6221
Pkg of 50, 9 cm high, 2 ml of prepacked analytical grade 1-X8 resin, 200–400 mesh, 45–106 particle size, formate form, includes 10 ml reservoir

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Poly-Prep® Columns, AG® 50W-X8, hydrogen form

731-6213
Pkg of 50, 9 cm high, 2 ml of prepacked analytical grade 50W-X8 resin, 100–200 mesh, 106–250 particle size, hydrogen form, includes 10 ml reservoir

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Poly-Prep® Columns, AG® 50W-X8, hydrogen form

731-6214
Pkg of 50, 9 cm high, 2 ml of prepacked analytical grade 50W-X8 resin, 200–400 mesh, 63–150 particle size, hydrogen form, includes 10 ml reservoir

List Price:   $230.00
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Poly-Prep® Column Stack Cap

731-1555
Pkg of 50, column stack cap, for use with Poly-Prep columns

List Price:   $41.00
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Number Description Options
LIT84 Instruction Manual, Prefilled Poly-Prep Columns for Ion Exchange Chromatography Click to download