Fluorescent Protein Stains

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Fluorescent protein gel staining, when combined with an appropriate imager, is a superior approach for protein analysis compared to Coomassie and silver staining. Fluorescent gel stains offer advantages such as broad linear range, higher sensitivity, and mass spectrometry compatibility. Bio-Rad offers three types of fluorescent protein gel stains, each optimized for your imager requirements:

Oriole™ fluorescent gel stain — An easy-to-use, fast, sensitive, and highly linear protein gel stain for visualization and quantitation of proteins in SDS-PAGE gels. The simple protocol requires a one-step staining process without prior fixing. Since destaining is not required, protein samples can be accurately visualized and quantitated in less than 2 hours. The excitation and emission characteristics of Oriole make it ideal for UV-based imagers.

Flamingo™ fluorescent gel stain — This 10x solution contains a novel dye that fluoresces when bound to denatured proteins. This easy-to-use gel stain can be used in both 1-D and 2-D electrophoretic applications and can detect down to 0.5 ng of protein. The staining procedure is a simple two-step protocol that can be completed in as little as 5 hours. The steps are not time sensitive and destaining is not required. Stained gels may be stored in the dark at 2–8°C for up to 6 months without significant loss of imaging sensitivity. Flamingo fluorescent gel stain is compatible with mass spectrometry. Gels stained with Flamingo fluorescent gel stain can also be visualized with a variety of fluorescence imaging systems, but it is particularly optimized for laser-based fluorescence scanners such as the Molecular Imager® PharosFX™ system.

SYPRO Ruby protein gel stain — The most widely used gel stain for 2-D electrophoresis, ready-to-use SYPRO Ruby protein gel stain is compatible with mass spectrometry and Edman-based sequencing applications. It can be visualized with UV or blue light transilluminators or laser scanners.

Oriole Fluorescent Gel Stain

161-0495
200 ml, 1x Oriole fluorescent gel stain solution

List Price:   $77.00
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Oriole Fluorescent Gel Stain

161-0496
1 L, 1x Oriole fluorescent gel stain solution

List Price:   $169.00
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Oriole Fluorescent Gel Stain, Kit

161-0497
5 L kit, materials sufficient for 5 L of 1x Oriole fluorescent stain solution

List Price:   $779.00
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Flamingo Fluorescent Gel Stain

161-0490
20 ml, 10x Flamingo fluorescent gel stain solution

List Price:   $63.00
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Flamingo™ Fluorescent Gel Stain

161-0491
100 ml, 10x Flamingo fluorescent gel stain solution

List Price:   $179.00
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Flamingo Fluorescent Gel Stain

161-0492
500 ml, 10x Flamingo fluorescent gel stain solution

List Price:   $855.00
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SYPRO Ruby Protein Gel Stain

170-3126
200 ml, 1x solution, ready-to-use fluorescent gel stain

List Price:   $85.00
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SYPRO Ruby Protein Gel Stain

170-3125
1 L, 1x solution, ready-to-use fluorescent gel stain

List Price:   $295.00
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SYPRO Ruby Protein Gel Stain

170-3138
5 L, 1x solution, ready-to-use fluorescent gel stain

List Price:   $1,019.00
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Number Description Options
5991 Oriole Fluorescent Gel Stain: A Novel Fluorescent Stain for a Simpler 2-D PAGE to MALDI Workflow, Rev A Click to download
2533 SYPRO Ruby Protein Gel Stain Flier, Rev E Click to download
5989 Imaging Fluorescently Stained Gels with Image Lab Software Quick Start Guide, Rev A Click to download
5705 Sensitivity and Protein-to-Protein Consistency of Flamingo Fluorescent Gel Stain Compared to Other Fluorescent Stains, Rev A Click to download
5346 Flamingo Fluorescent Gel Stain Product Information Sheet, Rev A Click to download
10003321 Instruction Manual, Flamingo Fluorescent Gel Stain, Rev A Click to download
4006173 Instruction Manual, SYPRO Ruby Protein Stains, Rev B Click to download
10017295 Instruction Manual, Oriole Fluorescent Gel Stain, Rev B Click to download
5900 Oriole Fluorescent Gel Stain PIS, Rev A Click to download
5754 Comparison of SYPRO Ruby and Flamingo Fluorescent Gel Stains With Respect to Compatibility With Mass Spectrometry, Rev A Click to download
5921 Oriole Fluorescent Gel Stain: Characterization and Comparison with SYPRO Ruby Gel Stain, Rev A. Click to download