Econo-Pac® Protein A Prepacked Gravity Flow Columns



Particle Size (µm) Capacity Applications
protein A
protein A gel
2 ml 10–14 mg
mouse IgG1*
10–14 mg
mouse IgG1*
Gravity-flow purification of monoclonal antibodies from small amounts of ascites fluid or serum.
protein A kit
300mg mouse
Contains everything necessary to purify monoclonal anitbodies from ascites fluid or serum, including Affi-Gel protein A column, reusable Econo-Pac 10DG columns (for a sample preparation and buffer exchange of the purified antibody), and MAPS II buffers. Enough reagents to purify 500 mg mouse IgG1.
serum IgG
DEAE Affi-Gel
blue gel
10 ml 75–150 3 ml serum per column per run For purification of IgG from other serum proteins and plasminogen with only residual transferrin contamination. Columns can be resued 8–10 times.
serum IgG
3 ml serum per column Contains everything necessary to purify IgG from serum, including 5 Econo-Pac serum IgG purification columns, reusable Econo-Pac 10DG column for sample preparation, and premixed buffers for human and rabbit IgG applications. Up to 15 ml serum can be purified at one time.
10DG desalting gel column
Bio-Gel® P-6 gel 10 ml 90–180 3 ml sample
per column per run
Gravity-flow column for desalting and buffer exchange applications. Exclusion limit is 6,000 Da.

* When used with MAPS II buffers.

Prepacked Cartridge Selection by Application
Cartridge by Application Support Type of Separation Chemical Form Protein capacity/ml*
Protein and Plasmid Purification
High Q Macro-Prep® High Q Strong anion exchange -N+(CH3)3 ≥37 mg BSA
UNOsphere Q UNOsphere™ Q Strong anion exchange -N+(CH3)3 180 mg BSA
UNOsphere S UNOsphere S Strong cation exchange -SO3- 60 mg human IgG
DEAE Macro-Prep DEAE Weak anion exchange -HN+(C2H5)2 >35 mg protein
Protein Purification
High S Macro-Prep High S Strong cation exchange -SO3- ≥49 mg human IgG
IgG Purification
DEAE Blue DEAE Affi-Gel® Blue Affinity Cibacron Blue
0.2–1.0 ml serum
Antibody Purification
Protein A Affi-Prep® protein A Affinity Protein A 8–10 mg mouse monoclonal IgG1
CHT™ Type I CHT ceramic
hydroxyapatite Type I
Hydroxyapatite (Ca5(PO4)3OH)2 16–23 mg human IgG
CHT Type II CHT ceramic
hydroxyapatite Type II
Hydroxyapatite (Ca5(PO4)3OH)2 25–60 mg human IgG
Affinity-Tagged Protein Purification
IMAC** Profinity™ IMAC Affinity Iminodiacetic acid ≥15 mg recombinant
His-tagged protein
GST** Profinity GST Affinity Glutathione ≥10 mg/ml recombinant
GST-tagged protein
Desalting, Buffer Exchange
P-6 Bio-Gel® P-6 Gel filtration Polyacrylamide 10–22% CV***
Purification of Serum Proteins, Enzymes
Blue Affi-Gel Blue Affinity Cibacron Blue
0.2 mlm serum
11 mg albumin

*   Protein capacity based on bulk support; check individual instruction manuals for run conditions and specifications.
**  IMAC and GST cartridges are designed for use with the Profinia protein purification system.
*** Column volume.

Product Selection by Technique
Packaging Format
Characteristics Product Type Analytical Pilot/Preparative Process Prepacked Bottled
Ion Exchange Chromatography
Separation by charge UNOsphere™ media     UNOsphere Q, S, Rapid S
General biomolecules purification Macro-Prep® media     Macro-Prep High Q, DEAE, High S, CM
Applicable to all antibody purifications Macro-Prep 25 media     Macro-Prep 25 Q, 25 S
UNO® columns   UNO Q, S  
Bio-Scale™ Mini Cartridges     UNOsphere Q, S  
High capacity         Macro-Prep High Q, S  
Good capture technique AG® resins   AG
Chelex® resins Chelex
Bio-Rex® resins Bio-Rex
Hydroxyapatite and Fluoroapatite Chromatography
Unique selectivity CHT™ 80 µm support     CHT, Types I and II
Alternative to protein A for antibody purification CHT 40 µm support     CHT, Types I and II
CHT 20 µm support   CHT, Types I and II
CFT 40 µm support CFT, Types I and II
Removal of leached protein A, aggregates, endotoxins, and DNA Bio-Gel® HT and HTP media     Bio-Gel HT, HTP
Bio-Scale columns   CHT Type I (10 µm)  
CHT Type II (20 µm)  
Affinity Chromatography
Purification of His- and GST- tagged proteins Profinity™ IMAC and GST media   Bio-Scale Mini Profinity IMAC
Separation by biological interaction Affi-Gel resins     Affi-Gel® Protein A*
Affi-Gel Blue*
DEAE Affi-Gel Blue*
CM Affi-Gel Blue*
High selectivity           Affi-Gel boronate, heparin**
High purity in a single step           Affi-Gel Hz, 10, 15**
Affi-Gel 102**
Excellent capture technique Profinity epoxide media     Profinity epoxide**
Affi-Prep media   Affi-Prep protein A*
Affi-Prep polymyxin*
Bio-Scale Mini cartridges   Profinity IMAC and GST  
Econo-Pac® columns   Affi-Gel Blue  
DEAE Affi-Gel Blue
Affi-Gel protein A
Size Exclusion Chromatography
Separation by size Bio-Gel P media     Bio-Gel P
MW determination Bio-Beads™ S-X media     Bio-Beads S-X
Applicable to all antibody purifications Bio-Sil® and Bio-Silect™ columns     Bio-Sil, Bio-Silect  
Desalting or buffer exchange Bio-Scale Mini cartridges     Bio-Gel P-6  
High resolution Econo-Pac columns     Econo-Pac 10DG  
Hydrophobic Interaction Chromatography
Separation by hydrophobicity Macro-Prep HIC media     Macro-Prep t-butly
Macro-Prep methyl

* Ready to use.
** Activated.

Econo-Pac prepacked columns for gravity-flow chromatography allow fast and simplified antibody purification. The prepacked Econo-Pac columns include an upper frit, a snap-off end tip, graduated column markings, and a 30 ml total column volume. To improve column performance, use a flow adaptor.

Econo-Pac Protein A Columns

Pkg of 5, prepacked gravity-flow chromatography columns, prefilled with Affi-Gel protein A gel

List Price:   $507.00
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Econo-Pac® Protein A Kit

Purification kit, includes Affi-Gel® protein A column, four 10DG desalting columns, buffers

List Price:   $307.00
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Econo-Pac Serum IgG Purification Kit

Antibody purification kit, includes 5 x 5 ml DEAE Affi-Gel Blue columns, 1 x 10 ml 10DG column, buffers

List Price:   $379.00
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Number Description Options
LIT154 Instruction Manual, Econo-Pac Serum IgG Purification Kit and Econo-Pac Serum IgG Purification Columns, Rev B Click to download
LIT153 Instruction Manual, Econo-Pac Protein A Kit, Protein A Columns, Rev B Click to download [ Add to Cart (Free) ]