C. elegans Behavior Kit

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Overview

Lab Preparation Checklist
Kit contains sufficient materials for 8 student workstations (2–4 students per workstation)
Store at –20°C  
E. coli OP50-pBAD, lyophilized 1
Cholesterol solution, 200 µl 1
Ampicillin, lyophilized, 30 mg 1
Store at room temperature  
NGM Lite Agar, 11 g 1
Agar, 4 g 1
2.5 M NaCl, 0.5 ml 1
C. elegans wash buffer (10x), 30 ml 1
Microcentrifuge tubes, 2.0 ml 60
Petri dishes, 60 mm 60
Disposable plastic transfer pipets (DPTPs) 50
Curriculum, (including Teacher's Guide, Student Manual, and graphic Quick Guide) 1
Redemption instructions for shipment of C. elegans 1
C. elegans, wild-type, 1 ml
C. elegans, mutant, 1 ml
 
Note: The wild-type and mutant worms are shipped on dry ice and must be kept on dry ice or at –70°C until ready to plate. The kit will be shipped prior to the shipment of worms to allow for preparation of NGM Lite agar plates. Wild-type and mutant C. elegans will be shipped overnight at date specified by customer.
Key Kit Features
  • Aligns with AP Biology Big Ideas 2, 3, and 4
  • Get hands-on experience with a model eukaryotic organism
  • Observe and study the life cycle of C. elegans
  • Utilize microscope skills
  • Learn about genetics and its effect on behavior
  • Student activities complete in two 45-minute lab sessions
C. elegans Videos

OpenWorm Resource



Learn about C. elegans' life cycle, their feeding habits, their neurological structure, and more.

C. elegans Movement



See C. elegans in motion and observe their movement patterns.

The C. elegans Behavior Kit allows students to work with and learn about C. elegans, one of the most studied model organisms.

Key Features and Benefits

With this kit, students are able to:

  • Get hands-on experience with a model eukaryotic organism
  • Observe and study the life cycle of C. elegans
  • Utilize their microscope skills
  • Learn how to subculture
  • Learn about genetics and its effect on behavior
  • Discover the connection between learning, the daf-18 gene, and the AIY and ASE neurons

Background Information

C. elegans was the first multicellular organism to have its complete genome sequenced. The Nobel Prize in Physiology or Medicine was awarded to C. elegans researchers in 2002 (genetics of organ development and apoptosis), 2006 (RNA interference), and 2008 (GFP expression), emphasizing the importance of research on this model organism. This microscopic nematode is ideal for students to learn about subculturing so that they can observe the life cycle and different stages of development of the worms.

Following life cycle observation, students will get the chance to understand how C. elegans can learn to associate salt with their food by monitoring chemotaxis. When wild-type C. elegans are fed food with salt they learn to associate their food with the salt. C. elegans are then able to sense salt in their environment and will chemotax towards the salt in search of food. A mutant affected in the daf-18 gene (and subsequently the AIY and ASE neurons) results in a worm that is not able to learn to associate the salt with the food. While the mutant C. elegans are able to chemotax, their inability to associate their food to a salty environment results in no migration towards salt in a chemotaxis assay.

Note: The wild-type and mutant worms are shipped on dry ice and must be kept on dry ice or at –70°C until ready to plate. Allow for preparation of NGM Lite agar plates (reagents ordered separately) prior to ordering the worms. Wild-type and mutant C. elegans will be shipped overnight at a date specified by the customer.

Download our interactive calculator for setting up your instructor's advance lab preparation and for scheduling shipment of the C. elegans.

Kit Contents Kit contains sufficient materials for 8 student workstations (2–4 students per workstation)
Store at –20°C  
E. coli OP50-pBAD, lyophilized 1
Cholesterol solution, 200 µl 1
Ampicillin, lyophilized, 30 mg 1
Store at room temperature  
NGM Lite agar, 11 g 1
Agar, 4 g 1
2.5 M NaCl, 0.5 ml 1
C. elegans wash buffer (10x), 30 ml 1
Microcentrifuge tubes, 2.0 ml 60
Petri dishes, 60 mm, sterile 60
Disposable plastic transfer pipets (DPTPs) 50
Curriculum, including Teacher's Guide, Student Manual, and graphic Quick Guide 1
C. elegans shipment redemption instructions 1
C. elegans, wild-type, 1 ml 1
C. elegans, mutant, 1 ml 1
Notes The wild-type and mutant worms are shipped on dry ice and must be kept on dry ice or at –70°C until ready to plate. The kit will be shipped prior to the shipment of worms to allow for preparation of NGM Lite agar plates. Wild-type and mutant C. elegans will be shipped overnight at date specified by customer.
C. elegans Behavior Kit

166-5120EDU
Pkg of 1, kit for C. elegans life cycle analysis and wild-type versus mutant behavior analysis; C. elegans shipped separately on requested date; education use only

List Price:   $218.75
EDU Price:   $175.00   What is this?
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C. elegans Behavior Kit RT Reagent Refill Pack

166-5121EDU
Pkg of 1, room temperature kit, includes NGM lite and assay agar, wash buffer, and salt solution; for use with the C. elegans behavior kit; education use only

List Price:   $56.25
EDU Price:   $45.00   What is this?
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C. elegans Behavior Kit TS Reagent Refill Pack

166-5123EDU
Pkg of 1, temperature-sensitive plastic bags, includes E. coli OP50-pBAD, cholesterol, and ampicillin; for use with the C. elegans behavior kit; education use only

List Price:   $56.25
EDU Price:   $45.00   What is this?
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C. elegans Behavior Kit, Wild-Type and Mutant

166-5122EDU
Pkg of 1, C. elegans behavior kit for wild-type and mutant worm strains; must be kept at –70°C or on dry ice until ready to plate; education use only

List Price:   $75.00
EDU Price:   $60.00   What is this?
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NGM Lite Agar

166-5125
Pkg of 1, 11 g, NGM lite agar for growth of wild-type and mutant C. elegans worm strains; for use with the C. elegans behavior kit; education use only

List Price:   $25.00
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Number Description Options
6673 Map of AP-Aligned Bio-Rad Kits with Investigative Laboratories, Rev A Click to download
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