ddPCR Gene Expression Assays

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ddPCR Gene Expression Assays enable accurate quantification of small fold changes in gene expression, for e.g. expression differences that are 2-fold or lower, for detecting targets that may be present at low levels of abundance or samples that contain substances that can inhibit a PCR reaction. ddPCR Gene Expression Assays are available as probe or EvaGreen Assays. Protocols are provided for use in One-Step or Two-Step RT-ddPCR protocols. Find and buy ddPCR Gene Expression Assays.

Key Benefits

• Enables detection of small fold changes in expression
• Choose from more than 60,000 gene expression assays for human, rat, and mouse
• Probe or EvaGreen® chemistry

Sample Data

Robust PrimePCR gene expression assays used in ddPCR with EvaGreen provide precise quantification of 18 reference genes. A, 1-D fluorescence amplitude plot shows results of a two-step reverse transcription ddPCR reference assay panel run on human brain RNA. The black clusters on the plot represent the negative droplets and the blue clusters represent the droplets that are positive for the respective reference assay. B, concentration plot shows various gene concentrations of cDNA calculated as copies/µl on a log10 scale. C, calculated RNA concentrations in the original human brain RNA sample for each reference gene. All error bars are generated by QuantaSoft software and represent the 95% confidence interval.

Packaging Options

ddPCR Gene Expression Probe Assays are available in multiple fluorophores. Reference assays with the complimentary fluorophore are available. These assays have been designed for maximum specificity and transcript coverage. Assays are available for human, mouse, and rat. Each tube contains 18uM primers and 5uM probe.

ddPCR Gene Expression Assays (EvaGreen) consists of unlabeled PCR primer pairs for use with dye-based chemistry such as the ddPCR EvaGreen Supermix. Individual assays are provided as a 20x stock solution. Each tube contains 5uM each primer.

Related Products

• iScript Advanced cDNA Synthesis Kit for RT-qPCR (Catalog# 172-5038)
• ddPCR Supermix for Probes no dUTP
• One-Step RT-ddPCR Advanced Kit for Probes