iScript™ Advanced cDNA Synthesis Kit, 100 x 20 µl rxns #1725038

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100 x 20 µl reactions, includes 400 µl 5x iScript Advanced Reaction Mix, 100 µl iScript Advanced Reverse Transcriptase

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Two-tube kit allows up to 7.5 &micro;g input RNA.</p> <ul> <li>Ability to use higher input RNA amounts to analyze a large number of target genes</li> <li>Detect low-level target genes &mdash; use with less input RNA, or when RNA sample is limited</li> <li>Reduce inter-assay variability and improve reproducibility &mdash; eliminate the need for multiple cDNA synthesis reactions</li> <li>Prevent 5&prime; and 3&prime; bias &mdash; optimum blend of oligo(dT) and random primers</li> <li>25 &micro;l (enough for 25 x 20 &micro;l reactions)</li> <li>2-tube kit generates cDNA in 21 min</li> </ul> <h2 class="descHeader">Packaging Options</h2> <div class="clear" style="height: 1px;">&nbsp;</div> <table class="pd_table pd_gridlines" border="0"> <tbody> <tr> <td align="left"><strong>Kit Size</strong></td> <td align="left"><strong>Catalog Number</strong></td> </tr> <tr class="pd_colorbackground"> <td align="left" valign="top">25 x 20 &micro;l reactions</td> <td align="left" valign="top"><a href="/en-us/sku/172-5037-iscript-advanced-cdna-synthesis-kit-for-rt-qpcr">172-5037</a></td> </tr> <tr> <td align="left" valign="top">100 x 20 &micro;l reactions</td> <td align="left" valign="top"><span style="font-size: 11px;">172-5038</span></td> </tr> </tbody> </table> <br /> <h2 class="descHeader">Related Products</h2> <ul> <li><a href="/evportal/destination/commerce/product_detail?catID=M87EVMKG4"><strong><span style="text-decoration: underline;">iScript&trade; Reverse Transcription Supermix for RT-qPCR</span></strong></a>: 1-tube format for total input 1 &micro;g&ndash;1 pg RNA, plus liquid at &ndash;20&deg;C, eliminating freeze-thaw cycles</li> <li><a href="/evportal/destination/commerce/product_detail?catID=M87EZXC4S"><strong><span style="text-decoration: underline;">iScript&trade; Select cDNA Synthesis Kit</span></strong></a>: synthesize long cDNAs up to 8 kb; oligo(dT), random primer, and gene-specific primer enhancer provided in separate tubes</li> <li><a href="/evportal/destination/commerce/product_detail?catID=M87EWZESH"><strong><span style="text-decoration: underline;">iScript&trade; cDNA Synthesis Kit</span></strong></a>: 2-tube format allows for up to 15 &micro;l of RNA sample in a 20 &micro;l reaction, in a 40 min protocol</li> </ul> <h2 class="descHeader">More Information</h2> <p>Learn about the <a href="/en-us/product/reverse-transcription-reagents/iscript-advanced-cdna-synthesis-kit-for-rt-qpcr?tab=Description">key features and specifications of the iScript&trade; Advanced cDNA Synthesis Kit for RT-qPCR.</a></p> /webroot/web/html/lsr/products/SKUs/autopopulated 172-5038-1.html /webroot/web/html/lsr/products/SKUs/autopopulated/172-5038-1.html 1 0 false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725038&CartQuote=QT&quantity= false true false $618.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725038&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725038&quantity= 170-8842-MSDS iScript Advanced cDNA Synthesis Kit RT-qPCR Australia English /webroot/web/pdf/WWMSDS/LSGC/AUS/AUS_ENG_1708842.pdf Korea Korean /webroot/web/pdf/WWMSDS/LSGC/ROK/ROK_KOR_1708842.pdf Belgium French /webroot/web/pdf/WWMSDS/LSGC/B/B_FR_1708842.pdf Italy Spanish /webroot/web/pdf/WWMSDS/LSGC/ISL/ISL_ES_1708842.pdf Canada French /webroot/web/pdf/WWMSDS/LSGC/FCN/FCN_FR_1708842.pdf The Netherlands Dutch /webroot/web/pdf/WWMSDS/LSGC/NL/NL_NL_1708842.pdf Hungary English /webroot/web/pdf/WWMSDS/LSGC/HE/HE_ENG_1708842.pdf Hungary Hungarian /webroot/web/pdf/WWMSDS/LSGC/H/H_UNG_1708842.pdf Germany German /webroot/web/pdf/WWMSDS/LSGC/D/D_D_1708842.pdf Czech Republic Czech /webroot/web/pdf/WWMSDS/LSGC/CZ/CZ_TS_1708842.pdf United Arab Emirates English /webroot/web/pdf/WWMSDS/LSGC/AE/AE_ENG_1708842.pdf Denmark Danish /webroot/web/pdf/WWMSDS/LSGC/DK/DK_DA_1708842.pdf Denmark English /webroot/web/pdf/WWMSDS/LSGC/DKE/DKE_ENG_1708842.pdf Germany English /webroot/web/pdf/WWMSDS/LSGC/DE/DE_ENG_1708842.pdf Poland Polish /webroot/web/pdf/WWMSDS/LSGC/PL/PL_POL_1708842.pdf Belgium English /webroot/web/pdf/WWMSDS/LSGC/BE/BE_ENG_1708842.pdf Hong Kong English /webroot/web/pdf/WWMSDS/LSGC/HK/HK_ENG_1708842.pdf Italy Italian /webroot/web/pdf/WWMSDS/LSGC/I/I_IT_1708842.pdf Portugal Portuguese /webroot/web/pdf/WWMSDS/LSGC/P/P_PT_1708842.pdf Canada English /webroot/web/pdf/WWMSDS/LSGC/CDN/CDN_USA_1708842.pdf USA English /webroot/web/pdf/WWMSDS/LSGC/USA/USA_USA_1708842.pdf true China Chinese /webroot/web/pdf/WWMSDS/LSGC/RC/RC_ZH_1708842.pdf Spain Spanish /webroot/web/pdf/WWMSDS/LSGC/E/E_ES_1708842.pdf Sweden Swedish /webroot/web/pdf/WWMSDS/LSGC/S/S_S_1708842.pdf Singapore English /webroot/web/pdf/WWMSDS/LSGC/SGP/SGP_ENG_1708842.pdf Czech Republic English /webroot/web/pdf/WWMSDS/LSGC/CZE/CZE_ENG_1708842.pdf United Kingdom English /webroot/web/pdf/WWMSDS/LSGC/GB/GB_ENG_1708842.pdf Austria English /webroot/web/pdf/WWMSDS/LSGC/AE/AE_ENG_1708842.pdf Sweden English /webroot/web/pdf/WWMSDS/LSGC/SE/SE_ENG_1708842.pdf France Hungarian /webroot/web/pdf/WWMSDS/LSGC/H/H_UNG_1708842.pdf France English /webroot/web/pdf/WWMSDS/LSGC/FE/FE_ENG_1708842.pdf New Zealand English /webroot/web/pdf/WWMSDS/LSGC/NZ/NZ_ENG_1708842.pdf Switzerland German /webroot/web/pdf/WWMSDS/LSGC/CH/CH_D_1708842.pdf France Czech /webroot/web/pdf/WWMSDS/LSGC/CZ/CZ_TS_1708842.pdf China English /webroot/web/pdf/WWMSDS/LSGC/PRC/PRC_ENG_1708842.pdf Italy English /webroot/web/pdf/WWMSDS/LSGC/IE/IE_ENG_1708842.pdf India English /webroot/web/pdf/WWMSDS/LSGC/IN/IN_ENG_1708842.pdf Switzerland English /webroot/web/pdf/WWMSDS/LSGC/CHE/CHE_ENG_1708842.pdf Korea English /webroot/web/pdf/WWMSDS/LSGC/KOR/KOR_ENG_1708842.pdf Finland Finnish /webroot/web/pdf/WWMSDS/LSGC/SF/SF_SF_1708842.pdf Israel English /webroot/web/pdf/WWMSDS/LSGC/IL/IL_ENG_1708842.pdf Poland English /webroot/web/pdf/WWMSDS/LSGC/POL/POL_ENG_1708842.pdf France French /webroot/web/pdf/WWMSDS/LSGC/F/F_FR_1708842.pdf The Netherlands English /webroot/web/pdf/WWMSDS/LSGC/NLE/NLE_ENG_1708842.pdf Austria German /webroot/web/pdf/WWMSDS/LSGC/A/A_D_1708842.pdf Switzerland French /webroot/web/pdf/WWMSDS/LSGC/CHF/CHF_FR_1708842.pdf Norway Norwegian /webroot/web/pdf/WWMSDS/LSGC/N/N_N_1708842.pdf
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Use the iScript Advanced cDNA Synthesis Kit for RT-qPCR for increased data throughput from a single 20 µl reverse transcription (RT) reaction for real-time qPCR. Two-tube kit allows up to 7.5 µg input RNA.

  • Ability to use higher input RNA amounts to analyze a large number of target genes
  • Detect low-level target genes — use with less input RNA, or when RNA sample is limited
  • Reduce inter-assay variability and improve reproducibility — eliminate the need for multiple cDNA synthesis reactions
  • Prevent 5′ and 3′ bias — optimum blend of oligo(dT) and random primers
  • 25 µl (enough for 25 x 20 µl reactions)
  • 2-tube kit generates cDNA in 21 min

Packaging Options

 
Kit Size Catalog Number
25 x 20 µl reactions 172-5037
100 x 20 µl reactions 172-5038

Related Products

More Information

Learn about the key features and specifications of the iScript™ Advanced cDNA Synthesis Kit for RT-qPCR.

Number Description Options
Available Translation
iScript Advanced cDNA Synthesis Kit RT-qPCR  (SDS)gene

Bulletin# 170-8842-MSDS

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