Traditional semi-dry transfer system transfers proteins ranging from 10–100 kD in 15–60 minutes. Trans-Blot Turbo system delivers efficient and reproducible transfer of proteins up to 400 kD in as little as 3 minutes.
Higher sensitivity and better transfer efficiency is seen with the Trans-Blot® Turbo™ system in comparison to other blotting techniques. This data set demonstrates the successful transfer of the 1.25 ng protein band only with the Trans-Blot Turbo system. Serial dilutions of transferrin were separated on a Criterion™ TGX™ 4–20% gel and transferred using four different blotting techniques. All images are adjusted to the same settings.
2x Protein Transfer
Quantitation performed on equivalent 2-D gels transferred with the Trans-Blot® Turbo™ and the iBlot systems demonstrated twice the number of proteins transferred and detected with the Trans-Blot Turbo system. Rat liver extract was focused on Bio-Rad's ReadyStrip™ 11 cm pH 5–8 IPG strips and separated on AnykD™ Criterion™ TGX™ gels. Duplicate gels were transferred with the Trans-Blot Turbo and iBlot systems, both using manufacturers’ recommended 7 min protocol. The nitrocellulose membranes were subsequently stained with SYPRO Ruby and imaged on a VersaDoc™ MP 4000 system.
Turbo Transfers with Trans-Blot® Turbo™ Transfer Packs
E. coli lysate (6 µg) was diluted two-fold. Samples were separated with Mini-PROTEAN TGX gels, transferred with the Trans-Blot Turbo system, stained with SYPRO Ruby and imaged on a VersaDoc™ MP 4000 system. Standards in lane 1 are Precision Plus Protein™ Unstained standards, with a top band of 250 kD MW.
Instructional video to guide you through the features, benefits, and operation of the Trans-Blot Turbo system.
Bio-Rad Trans-Blot SD Semi-Dry Blotting Apparatus
Trans-Blot SD Semi-Dry Assembly Guide
Call us at 1-800-4-BIORAD (1-800-424-6723)
Please reenter your email address in the correct format.
Please enter your email address.
Your subscription information already exists, we will send you an email with specific instructions to manage your existing subscription profile.
To receive the latest news, promotions, and more, sign up for Bio-Rad updates by entering your email address below. You can elect to receive only the types of Bio-Rad communications that are of interest to you..
Copyright © 2015 Bio-Rad Laboratories, Inc. All rights reserved.