UNO® Monolith Anion Exchange Columns

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Overview

SEM Image of Bed Matrix
Scanning electron micrograph showing the structure of the continuous-bed matrix (1,950x magnification). Note the nodulated structure of the polymer nodules and the large channels between them.
Purification on UNO® Column

Purification of phosphoinositide-
3-kinase on an UNO Q1 column.

Conditions
Buffer A, 40 mM TEA, pH 7.6
Buffer B, buffer A + 1 M KCl, pH 7.6
Gradient, 0–35% B in 20 ml
Flow rate, 2.5 ml/min

Data courtesy of Dr A Couvillon, Beth Israel Deaconess Medical Center, Boston, MA USA.

Flow Rate Comparison

Flow rate comparison for yeast enzyme separation.

Left, 1 ml/min; right, 5 ml/min. Separation on UNO® Q1 column in 20 mM Tris buffer, pH 8.2, with a gradient of 0–1 M NaCl in 20 ml.

UNO monolith ion exchange columns contain a patented* continuous-bed matrix allowing biomolecule separations at high flow rates without sacrificing resolution or capacity. In continuous-bed technology, advanced monomers and ionomers are polymerized directly in the column and aggregate into a dense homogeneous network of nodules. The resulting rigid matrix contains interconnecting channels large enough to allow fast hydrodynamic flow at low backpressures. The continuous-bed matrix yields a column with exceptional resolution, speed, capacity, and value for the separation of biomolecules. The homogeneous, nonporous continuous-bed matrix allows extremely fast mass transfer of proteins and eluent to the ionic functional groups.

UNO Q columns are for strong anion exchange. Choose from the following UNO columns:

  • UNO glass columns — provide high-resolution separation of biomolecules. The transparent glass tube allows easy bed inspection and column troubleshooting. Three column sizes provide flexibility for purification protocols
  • UNO replacement columns — provide a simple bed replacement as an alternative to purchasing a new column; three column sizes are available
  • UNO polishing PEEK columns — provides a late-stage purification tool used to obtain the highest resolution and recovery from small sample loads. The column allows you to purify and concentrate dilute samples in one step

* U.S. patent 6,423,666.

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UNO Q1 Column

7200001
Pkg of 1, 7 x 35 mm, 1.3 ml, prepacked monolith ion exchange column, 20 mg max. protein load, 0.5–5.0 ml/min flow rate, operating pressure 700 psi

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$2,046.00
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UNO Q6 Column

7200003
Pkg of 1, 12 x 53 mm, 6 ml, prepacked monolith ion exchange column, 90 mg max. protein load, 0.5–8.0 ml/min flow rate, operating pressure 700 psi

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$4,464.00
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UNO Q12 Column

7200005
Pkg of 1, 15 x 68 mm, 12 ml, prepacked monolith ion exchange column, 180 mg max. protein load, 0.5–8.0 ml/min flow rate, operating pressure 700 psi

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$6,618.00
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UNO Q Polishing Column

7200009
Pkg of 1, 4.6 x 10 mm, 0.16 ml, prepacked polishing PEEK column, 2 mg max. protein load, 0.1–1.0 ml/min flow rate, operating pressure 200 psi

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$708.00
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Number Description Options
6498 Lab-Scale Protein Purification – Column and Cartridge Selection Guide, Rev C Click to download [ Add to Cart (Free) ]
2352 Nucleotide and Oligonucleotide Separations on an UNO Q1 Ion Exchange Column Click to download
2304 Purification of rIL-13 Using a UNO® Q1 Column Click to download
2284 Purification of Two Isoforms of Pyruvate Kinase from Nordic Krill Using an UNO Q1 Column, Rev A Click to download
2283 Purification of the Components of Human Macular Carotenoid-Binding Complex, Rev A Click to download
2252 Purification of Elongation Factors From Xenopus laevis Oocytes Using UNO Q1 Column Click to download
4006085 Instruction Manual, UNO Column Bed Removal Tool, Rev A Click to download
4006084 Instruction Manual, UNO Q&S Continuous Bed Ion Exchange Replacement Column, Rev A Click to download
4006078 Instruction Manual, UNO® Q&S Polishing Column for Continuous Bed Ion Exchange, Rev A Click to download
4006076 Instruction Manual, UNO Q and S Continuous Bed Ion Exchange Columns Click to download