Each Bio-Rad real-time PCR system combines a thermal cycler with an optical reaction module for singleplex and multiplex detection of fluorophores. Every real-time PCR system features thermal gradient functionality.
Instrument Compatibility of PCR Plastic Consumables
Optimize Reactions in a Single Run
Determining the optimal temperature for primer annealing is crucial for efficient and specific amplification of product. With the CFX96 Touch™ system’s thermal gradient feature, you can determine the optimal temperature for primer annealing in a single experiment, minimizing the use of precious samples and reagents and saving valuable research time. At any step in a protocol, you can program a temperature gradient of up to 24°C across the reaction block. The thermal cycler provides exceptional temperature uniformity and reproducibility within each gradient zone, and the temperatures can easily be programmed and viewed onscreen in the software so you can quickly identify the optimal incubation temperature.
Thermal gradient experiment for optimizing annealing temperature. A tenfold dilution series (106 to 10 copies) of plasmid containing GAPDH template was amplified in the presence of SYBR® Green dye using a protocol with an annealing thermal gradient ranging from 55 to 68°C. Results are presented for three temperatures, showing 62°C as the optimal in this case, with early quantification cycle (Cq) values and the highest standard curve efficiency. RFU, relative fluorescence units.
This guidebook is a comprehensive source of information on real-time PCR. Complete with detailed information on optimizing, validating, and analyzing real-time PCR data, this technically focused applications guide is a resource for experienced researchers as well as those wanting to learn more about using real-time PCR for applications such as qPCR, RT-qPCR, genotyping, and GMO detection. This guidebook includes chapters that cover:
This guidebook comes with an easy-to-review real-time PCR quick guide to use at the bench. The quick guide covers the fundamentals of qPCR, design, optimization, and data analysis of qPCR experiments.
Over 20 Years of Innovation
From the introduction of the PTC-100® in 1988 to the launch of the C1000 Touch™ thermal cycler in 2011, Bio-Rad Laboratories has led the way in thermal cycler innovation for over 20 years. Bio-Rad's unique family of products for PCR research gives you the power to do the experiments you want to do, the way you want to do them.
The CFX96™ optical reaction module converts a C1000 Touch™ thermal cycler into a real-time PCR system. This six-channel system combines advanced optical technology with precise thermal control.
The CFX384 Touch real-time PCR system brings flexibility and ease of use to researchers performing precise, high-throughput real-time PCR applications in a 384-well format.
The CFX Connect real-time PCR system provides excellent thermal performance for two-target multiplex PCR and routine detection of single fluorophore experiments.
The CFX96 Touch Deep Well real-time PCR system offers industry-leading technology for large-volume reactions in real-time PCR applications.
The CFX automation system works with the CFX96 Touch™ and CFX384 Touch™ real-time PCR systems to facilitate the automation of workflows, generation of large volumes of data, and rapid data analysis.
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