iScript™ gDNA Clear cDNA Synthesis Kit, 500 x 20 µl reactions #1725035BUN



500 x 20 µl reactions, 5 x 400 µl 5x supermix, contains reverse transcriptase, RNase inhibitor, dNTPs, primers, MgCl2, stabilizers; 5 x 400 µl no-RT control supermix; 5 x 50 µl DNase; 5 x 150 µl DNase buffer



Use iScript gDNA Clear cDNA Synthesis Kit for easy and rapid generation of qPCR-ready cDNA free of genomic DNA (gDNA) for accurate gene expression analysis by real-time PCR.

Features and Benefits

  • Effective gDNA removal in purified RNA samples
  • Proprietary DNase buffer for maintaining RNA integrity during gDNA removal
  • Convenient all-in-one, single-tube RT supermix
  • Ready-to-use no-RT control supermix for monitoring gDNA contamination
  • gDNA-free cDNA ready in 36 min

gDNA-Free cDNA Ready in 36 Minutes

This kit allows you to effectively remove contaminating gDNA and reliably synthesize cDNA with minimal hands-on time in an easy-to-follow protocol, resulting in cDNA free of genomic DNA in 36 minutes.

Workflow for DNA Removal and cDNA Synthesis

Packaging Options

Kit Size Catalog Number
25 x 20 µl reactions 1725034
100 x 20 µl reactions 1725035
500 x 20 µl reactions 1725035BUN

Supporting Documentation

Download the iScript gDNA Clear cDNA Synthesis Kit Product Insert for detailed information about step-by-step protocols for cDNA synthesis and troubleshooting tips.

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