Image
Overview
25 x 20 µl reactions, 100 µl 5x supermix, contains reverse transcriptase, RNase inhibitor, dNTPs, primers, MgCl2, stabilizers; 200 µl no-RT control supermix; 12.5 µl DNase; 150 µl DNase buffer
Safety Data Sheet
Description
Description
Use iScript gDNA Clear cDNA Synthesis Kit for easy and rapid generation of qPCR-ready cDNA free of genomic DNA (gDNA) for accurate gene expression analysis by real-time PCR.
Features and Benefits
- Effective gDNA removal in purified RNA samples
- Proprietary DNase buffer for maintaining RNA integrity during gDNA removal
- Convenient all-in-one, single-tube RT supermix
- Ready-to-use no-RT control supermix for monitoring gDNA contamination
- gDNA-free cDNA ready in 36 min
gDNA-Free cDNA Ready in 36 Minutes
This kit allows you to effectively remove contaminating gDNA and reliably synthesize cDNA with minimal hands-on time in an easy-to-follow protocol, resulting in cDNA free of genomic DNA in 36 minutes.
Packaging Options
| Kit Size | Catalog Number |
| 25 x 20 µl reactions | 1725034 |
| 100 x 20 µl reactions | 1725035 |
| 500 x 20 µl reactions | 1725035BUN |
Supporting Documentation
Download the iScript gDNA Clear cDNA Synthesis Kit Product Insert for detailed information about step-by-step protocols for cDNA synthesis and troubleshooting tips.
Related Products
Related Categories
Specifications
Specifications
Product expiration
(after shipping date)
(after shipping date)
12 months
Storage
Store DNase buffer at 4°C upon receipt. Store all other kit components at –20°C.
Recommended SKUs
Recommended SKUs
Image
1708840
25 x 20 µl reactions, 100 µl 5x supermix, contains reverse transcriptase, RNase inhibitor, dNTPs, primers, MgCl2, stabilizers; 50 reactions of no-RT control supermix