Teach your students about transfection with a high rate of success. When paired with GFP lessons and ZOE class activities, you can simultaneously explain the principles of fluorescent imaging.
Use the general purpose TransFectin Lipid Reagent for high-efficiency transfection of your cell lines or primary cells, including difficult-to-transfect cells.
The proprietary mixture of cationic compound and the co-lipid DOPE (1,2-dioleoyl-sn-glycero-3-phosphoethanolamine) is designed to provide high expression levels with reduced cytotoxicity.
- Sufficient for 60 to 100 cell transfections in 35 mm plates
- Simple 3-step protocol gives high-efficiency transfection in a broad range of cells with high levels of cell viability
- Transfection at culture densities from 40% to 90%
- Designed to work in media containing serum but can be used in the absence of serum
- No post transfection media changes required
- Polystyrene is recommended because cationic lipid-plasmid complexes may bind to polypropylene
The Transfection Protocol Online Library features a comprehensive list of cell lines and primary cultures and contains protocols from a range of sources for transfection using lipids, electroporation, and biolistic particle delivery.
|Volume||Number of Vials||Catalog Number|
|0.5 ml||1 x 0.5 ml||1703350EDU|
|1 ml||1 x 1 ml||1703351EDU|
|5 ml||5 x 1 ml||1703352EDU|
- CFDA-SE Dye to monitor cell health
- Fixable Viability Dyes to distinguish between live and dead cells
- PureBlu™ DAPI and PureBlu Hoechst 33342 Nuclear Staining Dye to visualize nuclei
- siLentFect™ Lipid Reagent for RNAi for delivery of siRNA alone or cotransfected with a small reporter-plasmid
- SmartSpec™ Plus Spectrophotometer
- ZOE™ Fluorescent Cell Imager
- Gene Transfer/Transfection
- Biolistic Particle Delivery Systems
- Nucleic Acid Extraction and Purification
- PCR Instrumentation
- PCR Amplification Reagents
- Fluorescent Cell Imaging Activities for Your Classroom Flier
- TransFectin Lipid Reagent Brochure
- TransFectin Lipid Reagent Instruction Manual
- Optimization of TransFectin Lipid Reagent-Mediated Transfection for Different Cell Types Bulletin
- Transfection Reagent References