Overview
5,000 x 20 µl reactions, 50 ml (1 x 50 ml) 2x one-step reaction mix containing iTaq DNA polymerase, buffers, and ROX normalization dyes; 2.5 ml (1 x 2.5 ml) iScript reverse transcriptase; and 45 ml (1 x 45 ml) nuclease-free water
Safety Data Sheet
Description
Description
Use our combined iScript RNase H+ reverse transcriptase and antibody hot-start iTaq DNA polymerase probe kit formulation to complete your real-time reactions in one step — PCR efficiency is improved over a wide dynamic range and under various conditions.
Key Features and Benefits
- Sensitive and reproducible singleplex or multiplex reactions directly from RNA
- Advanced formulation for amplifying difficult target sequences and challenging samples
- Tested with ethanol for inhibitor tolerance and compatible with up to 4 µl crude lysate
- Works on any real-time PCR system
Kit Components
- Reverse transcriptase — 2.5 ml (1 x 2.5 ml)
- Reaction mix — 50 ml (1 x 50 ml)
- Nuclease-free water — 45 ml (1 x 45 ml)
Instrument Compatibility
iTaq Universal Probes One-Step RT-qPCR Kit is compatible with Bio-Rad Real-Time PCR Detection Systems and commercially available systems listed below:
| Systems | |
| Applied Biosystems |
|
| Stratagene |
|
| Eppendorf |
|
| QIAGEN |
|
| Roche |
|
| BioFire |
|
* BSA must be added according to instrument specifications
Applications and Uses of the iTaq Universal Probes One-Step Kit
- qPCR/real-time PCR
- Gene expression analysis
- Multiplexing
- Absolute quantification
- Mutation detection
- Pathogen detection
- Viral detection (load)
- Genetically modified organisms (GMO) characterization
- Genetic profiling
Packaging Options
The iTaq universal probes one-step kit is also available in a format of 100 reactions (1725140) and 500 reactions (1725141).
Related Products
Bio-Rad has also developed one-step kits for SYBR®Green specific applications.
Specifications
Specifications
Product format
Two-tube system
Kit content
iScript reverse transcriptase + 2x iTaq PCR reaction mix
Detection chemistry
SYBR Green or probes
Multiplex capability (for probes only)
Duplex (triplex possible depending on the abundance of the targets)
Inhibitor tolerance
Tested with ethanol. Also compatible with up to 4 µl of crude lysates
5’ to 3’ exonuclease activity of the enzyme(s)
Yes
Hot-start, activation time
Yes, 1–3 min
Sample input
100 fg to 100 ng
Sensitivity
10 copies in a duplex reaction
Reference dye
Patented universal dye preblended in the iTaq reaction mix
Instrument compatibility
Both ROX-dependent (high- and low-) and -independent instruments
Product expiration (after shipping date)
12 months
RT storage at –20°C (after shipping date)
12 months
Reaction mix storage at 4°C (after thawing)
Up to 3 months
Customer guarantee (minimum usage time) at –20°C
12 months