iScript™ cDNA Synthesis Kit

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Overview

Offers ease-of-use and a linear dynamic range of 1 µg to 100 fg. This two-component cDNA synthesis kit uses a 26-minute protocol.
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Description

Description

The iScript cDNA synthesis kit is a sensitive and easy-to-use first-strand cDNA synthesis kit for gene expression analysis using real-time qPCR. This two-tube kit is optimized to yield sensitive, unbiased representation over a broad dynamic range, with minimal setup and reaction time.

Key Features and Benefits

  • Have ease of use and consistency — 2-tube (iScript reaction mix and iScript reverse transcriptase) format for faster setup
  • Detect low-level target genes and conserve RNA during gene expression analysis — broad linear dynamic range of total input RNA (1 µg–1 pg) with a highly efficient RNase H+ MMLV reverse transcriptase
  • Utilize dilute RNA samples — reaction mix and iScript enzyme blend format allows up to 15 µl of RNA sample in a 20 µl cDNA synthesis reaction
  • Prevent 5' and 3' bias of your target genes and design primers in any region — optimum blend of oligo(dT) and random primers in the 5x supermix
  • Avoid skewed data from RNA degradation — potent blend of RNaseA inhibitor protects RNA during setup and reverse transcription
  • Complete cDNA synthesis and qPCR on the same day — fast 26 minute cDNA synthesis protocol

Specifications

Specifications

Storage at –20°C
Guaranteed for 12 months at –20°C in a constant temperature freezer (multiple freeze thaws not recommended)

Ordering

Ordering

items

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1708890
25 x 20 µl reactions, includes 5x reverse-transcription reaction mix, iScript reverse transcriptase, nuclease-free water
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1708891
100 x 20 µl reactions, includes 5x reverse-transcription reaction mix, iScript reverse transcriptase, nuclease-free water
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1708891BUN
500 x 20 µl reactions, includes 5x reverse-transcription reaction mix, iScript reverse transcriptase, nuclease-free water

Documents

Documents

Number Description Options
4106228
iScript™ cDNA Synthesis Kit Product Insert, Ver D
6746
iScript Reverse Transcription Kits Flier
[ Add to Cart (Free) ]
6252
Reagent Comparison Guide for Real-Time PCR Brochure, Rev C
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5990
Gene Expression Analysis Solutions Brochure
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6090
Amplification Reagents and Plastics Brochure
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6763
Gene Expression Real-Time PCR Workflow Flier
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