Description
Description
This digital PCR supermix for probes is a 2x concentrated, ready-to-use universal mix that has been optimized to deliver maximum PCR efficiency, specificity, and sensitivity in Droplet Digital PCR (ddPCR).
This supermix is suitable for use with UNG decontamination protocols.
Key Benefits
- Contains all components required for hydrolysis probe–based ddPCR except primers, probe(s), and template
- Limits nonspecific PCR amplification
- Hot-start polymerase keeps enzyme inactive during sample droplet partitioning
Applications and Uses
- Absolute quantification
- Mutation detection
- Copy number analysis
- Gene expression analysis
Packaging Options
| Number of 20 µl Reactions | Supermix Volume, ml | Catalog Number |
|---|---|---|
| 200 | 2 x 1 | 186-3026 |
| 500 | 5 x 1 | 186-3010 |
| 2,500 | 5 x 5 | 186-3027 |
| 5,000 | 10 x 5 | 186-3028 |
Additional Reagents
Bio-Rad offers additional consumables for completing your digital PCR workflow:
Additional Equipment
These digital PCR assays require the use of the following instruments and accessories:
- QX200 Droplet Generator
- QX200 Droplet Reader
- PX1 PCR Plate Sealer
- PTC Tempo Deepwell Thermal Cycler or C1000 Touch Thermal Cycler with 96 Deep–Well Reaction Module
More Information
The ddPCR Supermix for Probes (no dUTP) is optimized for use with Droplet Generation Oil for Probes on the QX600 or QX200 Droplet Digital PCR System.
Specifications
Specifications
Ordering
Ordering
items
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2 ml (2 x 1 ml), 2x supermix, for use in sample preparation for droplet generation in the QX600/QX200 Droplet Digital™ PCR Systems
5 ml (5 x 1 ml), 2x supermix, for use in sample preparation for droplet generation in the QX600/QX200 Droplet Digital™ PCR Systems
25 ml (5 x 5 ml), 2x supermix, for use in sample preparation for droplet generation in the QX600/QX200 Droplet Digital™ PCR Systems
50 ml (10 x 5 ml), 2x supermix, for use in sample preparation for droplet generation in the QX600/QX200 Droplet Digital™ PCR Systems
Accessories
Accessories
70 ml (10 x 7 ml), oil for use with droplet generator in the QX600/QX200 Droplet Digital™ PCR Systems
2 L (2 x 1 L), oil for use with droplet reader in the QX600/QX200 Droplet Digital™ PCR Systems
Pkg of 24, cartridges for use with the QX200 Droplet Generator
Pkg of 24, gaskets for use with QX200 Droplet Generator
5 pkg of 24 DG8 Cartridges, 5 pkg of 24 DG8 Gaskets, for use with the QX200 Droplet Generator
Equipment
Equipment
Droplet Digital PCR System, includes droplet generator, droplet reader, computer, software, associated component consumables, for EvaGreen or probe-based digital PCR applications
Droplet generator for EvaGreen or probe-based digital PCR applications, for use with QX200™ Droplet Digital™ PCR System
Droplet reader for EvaGreen or probe-based digital PCR applications, for use with the QX200 Droplet Digital™ System
Modular thermal cycler platform, includes C1000 Touch Thermal Cycler chassis, 96–deep well reaction module, USB flash drive