ddPCR™ Supermix for Probes

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Overview

This ready-to-use digital PCR supermix for probes is ideal for use with uracil N-glucosylase (UNG) decontamination protocols to prevent the reamplification of carryover PCR products between experiments.

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Description

Description

This digital PCR supermix for probes is a 2x concentrated, ready-to-use universal mix that has been optimized to deliver maximum PCR efficiency, specificity, and sensitivity in Droplet Digital PCR (ddPCR).

This supermix is suitable for use with UNG decontamination protocols.

Key Benefits

  • Contains all components required for hydrolysis probe–based ddPCR except primers, probe(s), and template
  • Limits nonspecific PCR amplification
  • Hot-start polymerase keeps enzyme inactive during sample droplet partitioning

Applications and Uses

  • Absolute quantification
  • Mutation detection
  • Copy number analysis
  • Gene expression analysis

Packaging Options

Number of 20 µl Reactions Supermix Volume, ml Catalog Number
200 2 x 1 186-3026
500 5 x 1 186-3010
2,500 5 x 5 186-3027
5,000 10 x 5 186-3028

 

Additional Reagents

 

Bio-Rad offers additional consumables for completing your digital PCR workflow:

Additional Equipment

These digital PCR assays require the use of the following instruments and accessories:

More Information

The ddPCR Supermix for Probes (no dUTP) is optimized for use with Droplet Generation Oil for Probes on the QX200 Droplet Digital PCR System.

Specifications

Specifications

Storage at –20°C
Up to 18 months (refer to expiration date)
Storage at 4°C (after thawing)
Up to 2 weeks
Template compatibility
cDNA, genomic DNA, plasmid DNA

Ordering

Ordering

items

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qx100-ddpcr-supermix-probes-2ml-186-3026-thumb.jpg
1863026
2 ml (2 x 1 ml), 2x supermix, for use in sample preparation for droplet generation in the QX200™/QX100™ Droplet Digital™ PCR Systems
qx100-ddpcr-supermix-probes-5ml-186-3010-tn.jpg
1863010
5 ml (5 x 1 ml), 2x supermix, for use in sample preparation for droplet generation in the QX200™/QX100™ Droplet Digital™ PCR Systems
qx100-ddpcr-supermix-probes-25ml-186-3027-tn.jpg
1863027
25 ml (5 x 5 ml), 2x supermix, for use in sample preparation for droplet generation in the QX200™/QX100™ Droplet Digital™ PCR Systems
qx100-ddpcr-supermix-probes-50ml-186-3028-thumb.jpg
1863028
50 ml (10 x 5 ml), 2x supermix, for use in sample preparation for droplet generation in the QX200™/QX100™ Droplet Digital™ PCR Systems

Accessories

Accessories

qx100-droplet-generator-oil-186-3005-thumb.jpg
1863005
70 ml (10 x 7 ml), oil for use with droplet generator in the QX200/QX100™ Droplet Digital™ PCR Systems
qx100-ddpcr-buffer-control-kit-186-3052-thumb.jpg
1863052
9 ml (2 x 4.5 ml), 2x buffer, for use as blank control with Droplet Generation Oil for Probes
qx100-droplet-reader-oil-2l-186-3004-thumb.jpg
1863004

2 L (2 x 1 L), oil for use with droplet reader in the QX200™/QX100™ Droplet Digital™ PCR Systems

dg8-cartridges-for-qx100-qx200-droplet-generator-186-4008-thumb.jpg
1864008
Pkg of 24, cartridges for use with the QX200™/QX100™ Droplet Generator
qx100-dg8-gaskets-186-3009-thumb.jpg
1863009
Pkg of 24, gaskets for use with QX200™/QX100™ Droplet Generator
droplet-generator-cartridges-and-gaskets-186-4007-thumb.jpg
1864007
5 pkg of 24 DG8 Cartridges, 5 pkg of 24 DG8 Gaskets, for use with the QX200™/QX100™ Droplet Generator
pierceable-foil-heat-seal-181-4040-thumb.jpg
1814040
Pkg of 100, foil seals for PCR and QX200/QX100 Droplet Digital PCR System applications, for use with PX1 PCR Plate Sealer

Equipment

Equipment

qx200-ddpcr-system-186-4001-thumb.jpg
1864001

Droplet Digital PCR System, includes droplet generator, droplet reader, computer, software, associated component consumables, for EvaGreen or probe-based digital PCR applications

qx200-droplet-generator-186-4002-thumb.jpg
1864002

Droplet generator for EvaGreen or probe-based digital PCR applications, for use with QX200™ Droplet Digital™ PCR System

qx200-droplet-reader-186-4003-thumb.jpg
(Discontinued) QX200 Droplet Reader
1864003
Droplet reader for EvaGreen or probe-based digital PCR applications, for use with the QX200 Droplet Digital™ System
PX1-PCR-Plate-Sealer-SKU-181-4000-thumb.jpg
(Discontinued) PX1 PCR Plate Sealer
1814000
PCR plate sealer, includes heat sealing instrument, plate support block that holds 96-well and 384-well plates, sealing frame, power cord
185-1196_tn.jpg
1851197

Modular thermal cycler platform, includes C1000 Touch Thermal Cycler chassis, 96–deep well reaction module, USB flash drive

Documents

Documents

Number Description Options
6338
Droplet Digital™ PCR (ddPCR™) Supermixes Flier, Rev A
[ Add to Cart (Free) ]
10026235
ddPCR™ Supermix for Probes Product Insert
10040718
Droplet Generation Oil for Probes Product Insert
6311
QX200 Droplet Digital PCR System Brochure, Rev C
[ Add to Cart (Free) ]
10031907
Instruction Manual, QX200™ Droplet Generator, Rev C
10031906
Instruction Manual, QX200 Droplet Reader and QuantaSoft Software