This digital PCR supermix for probes is a 2x concentrated, ready-to-use universal mix that has been optimized to deliver maximum PCR efficiency, specificity, and sensitivity in Droplet Digital PCR (ddPCR).
This supermix is suitable for use with UNG decontamination protocols.
- Contains all components required for hydrolysis probe–based ddPCR except primers, probe(s), and template
- Limits nonspecific PCR amplification
- Hot-start polymerase keeps enzyme inactive during sample droplet partitioning
Applications and Uses
- Absolute quantification
- Mutation detection
- Copy number analysis
- Gene expression analysis
|Number of 20 µl Reactions||Supermix Volume, ml||Catalog Number|
|200||2 x 1||186-3026|
|500||5 x 1||186-3010|
|2,500||5 x 5||186-3027|
|5,000||10 x 5||186-3028|
Bio-Rad offers additional consumables for completing your digital PCR workflow:
These digital PCR assays require the use of the following instruments and accessories:
- QX200 Droplet Generator
- QX200 Droplet Reader
- PX1 PCR Plate Sealer
- C1000 Touch Thermal Cycler with 96 Deep–Well Reaction Module
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Droplet Digital PCR System, includes droplet generator, droplet reader, computer, software, associated component consumables, for EvaGreen or probe-based digital PCR applications
Modular thermal cycler platform, includes C1000 Touch Thermal Cycler chassis, 96–deep well reaction module, USB flash drive