C. elegans Behavior Kit

C. elegans Behavior Kit

Overview

Compare a wild-type strain of C. elegans to a neurologic mutant to understand how a loss of the AIY and ASE neurons impacts learning capacity through a chemotaxis experiment.
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Ordering Information

Description

Description

The C. elegans Behavior Kit allows students to work with and learn about C. elegans, one of the most studied model organisms.

Key Features and Benefits

With this kit, students are able to:

  • Get hands-on experience with a model eukaryotic organism
  • Observe and study the life cycle of C. elegans
  • Utilize their microscope skills
  • Learn how to subculture
  • Learn about genetics and its effect on behavior
  • Discover the connection between learning, the daf-18 gene, and the AIY and ASE neurons

Background Information

C. elegans was the first multicellular organism to have its complete genome sequenced. The Nobel Prize in Physiology or Medicine was awarded to C. elegans researchers in 2002 (genetics of organ development and apoptosis), 2006 (RNA interference), and 2008 (GFP expression), emphasizing the importance of research on this model organism. This microscopic nematode is ideal for students to learn about subculturing so that they can observe the life cycle and different stages of development of the worms.

Following life cycle observation, students will get the chance to understand how C. elegans can learn to associate salt with their food by monitoring chemotaxis. When wild-type C. elegans are fed food with salt they learn to associate their food with the salt. C. elegans are then able to sense salt in their environment and will chemotax towards the salt in search of food. A mutant affected in the daf-18 gene (and subsequently the AIY and ASE neurons) results in a worm that is not able to learn to associate the salt with the food. While the mutant C. elegans are able to chemotax, their inability to associate their food to a salty environment results in no migration towards salt in a chemotaxis assay.

Note: The wild-type and mutant worms are shipped on dry ice and must be kept on dry ice or at –70°C until ready to plate. Allow for preparation of NGM Lite agar plates (reagents ordered separately) prior to ordering the worms. Wild-type and mutant C. elegans will be shipped overnight at a date specified by the customer.

Download our interactive calculator for setting up your instructor's advance lab preparation and for scheduling shipment of the C. elegans.

Specifications

Specifications

Kit Contents
Kit contains sufficient materials for 8 student workstations (2–4 students per workstation)
Store at –20°C
 
E. coli OP50-pBAD, lyophilized
1
Cholesterol solution, 200 µl
1
Ampicillin, lyophilized, 30 mg
1
Store at room temperature
 
NGM Lite agar, 11 g
1
Agar, 4 g
1
2.5 M NaCl, 0.5 ml
1
C. elegans wash buffer (10x), 30 ml
1
Microcentrifuge tubes, 2.0 ml
60
Petri dishes, 60 mm, sterile
60
Disposable plastic transfer pipets (DPTPs)
50
Curriculum, including teacher's guide, student manual, and graphic quick guide
1
C. elegans shipment redemption instructions
1
C. elegans, wild-type, 1 ml
1
C. elegans, mutant, 1 ml
1
Notes
The wild-type and mutant worms are shipped on dry ice and must be kept on dry ice or at   –70°C until ready to plate. The kit will be shipped prior to the shipment of worms to allow for preparation of NGM Lite agar plates. Wild-type and mutant C. elegans will be shipped overnight at date specified by customer.

Ordering

Ordering

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(Discontinued) C. elegans Behavior Kit
1665120EDU
Pkg of 1, kit for C. elegans life cycle analysis and wild-type versus mutant behavior analysis; C. elegans shipped separately on requested date (wild-type and mutant C. elegans worms (166-5122EDU) are not available in Europe; please order these separately from another supplier); education use only
c-elegans-behavior-kit-rt-reagent-refill-pack-166-5121edu-thumb.jpg
(Discontinued) C. elegans Behavior Kit RT Reagent Refill Pack
1665121EDU
Pkg of 1, room temperature kit, includes NGM lite and assay agar, wash buffer, and salt solution; for use with the C. elegans behavior kit; education use only
c-elegans-behavior-kit-ts-reagent-refill-pack-166-5123edu-thumb.jpg
(Discontinued) C. elegans Behavior Kit TS Reagent Refill Pack
1665123EDU
Pkg of 1, temperature-sensitive plastic bags, includes E. coli OP50-pBAD, cholesterol, and ampicillin; for use with the C. elegans behavior kit; education use only
c-elegans-behavior-kit-wild-type-and-mutant-166-5122edu-thumb.jpg
(Discontinued) C. elegans Behavior Kit, Wild-Type and Mutant
1665122EDU
Pkg of 1, C. elegans behavior kit for wild-type and mutant worm strains; must be kept at –70°C or on dry ice until ready to plate; education use only
ngm-lite-agar-166-5125edu-thumb.jpg
(Discontinued) NGM Lite Agar
1665125EDU
Pkg of 1, 11 g, NGM lite agar for growth of wild-type and mutant C. elegans worm strains; for use with the C. elegans behavior kit; education use only

Accessories

Accessories

1660508EDU_tn
(Discontinued) Professional Adjustable-Volume 100–1,000 μl Digital Micropipet
1660508EDU

Pkg of 1, 100–1,000 μl adjustable-volume micropipet, includes digital dial micrometer, tip ejector, accommodates standard pipet tips, fully autoclavable; education use only

166-0508BLK_tn.jpg
(Discontinued) Professional Adjustable-Volume 100–1,000 μl Digital Micropipet
1660508BLK
Pkg of 8,100–1,000 μl adjustable-volume micropipet, includes digital dial micrometer, tip ejector, accommodates standard pipet tips, fully autoclavable; education use only
166-0553EDU_tn.jpg
(Discontinued) Classroom 100–1,000 μl Digital Micropipet
1660553EDU
Pkg of 1, 100–1,000 μl, adjustable-volume micropipet, includes digital dial, tip ejector, accommodates standard pipet tips; education use only
166-0553BLK_tn.jpg
(Discontinued) Classroom 100–1,000 μl Digital Micropipet, Set of 8
1660553BLK
Pkg of 8,100–1,000 μl, adjustable-volume micropipet, includes digital dial, tip ejector, accommodates standard pipet tips; education use only
166-0487_tn.jpg
(Discontinued) Carousel Micropipet Rack
1660487EDU
Pkg of 1, benchtop rack, holds up to 6 single-channel professional micropipets, for use only with professional micropipets; education use only
166-0486EDU_tn.jpg
(Discontinued) Professional Micropipet Backpack Starter Set
1660486EDU

Starter set, includes 4 professional micropipets (0.5–10 μl, 2–20 μl, 20–200 μl, 100–1,000 μl), carousel micropipet rack, 4 racks of pipet tips (TBR-14, -35, -40, Prot/Elec), Bio-Rad backpack (style may vary); for education use only

Documents

Documents

Number Description Options
6673
Map of AP-Aligned Bio-Rad Kits with Investigative Laboratories
6672
Map of AP-Aligned Bio-Rad Kits with Essential Knowledge
6605
Map of AP-Aligned Bio-Rad Kits with Learning Objectives
10040890
C. elegans Behavior Kit Chi Square Analysis Supplement, Rev A
10041144
C. elegans Behavior Kit Neurology Supplement, Rev A
10030940
Instruction Manual, Biotechnology Explorer™ C. elegans Behavior Kit, Rev B
10033926
Biotechnology Explorer™ C. elegans Behavior Kit Planning Guide, Rev A
D102454
Bio-Rad Explorer™ C. elegans Behavior Kit Quick Guide, Rev B
10040011
Instruction Manual, Biotechnology Explorer™ C. elegans Behavior Kit, Bioinformatics Supplement, Rev A
7184
Response to Environment AP Strand Poster, Ver A