Biofuel Enzyme Reactions Kit for AP Biology

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Overview

Explore enzyme kinetics associated with the production of cellulosic ethanol, a biofuel. This ThINQ! Kit provides support for an open or guided inquiry format, enabling a student-driven learning approach. Covers Big idea 2 and 4.

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Description

Description

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Enzyme Lab Activity for AP Biology and Biotechnology Classrooms

The Biofuel Enzyme Reactions Kit for AP Biology is a student-driven, inquiry-based kit that characterizes the activity of the cellobiase enzyme. In this ThINQ! Investigation, students will play the role of bioengineer to explore the power of enzyme kinetics and optimize conditions that will maximize the rate of sugar conversion for the production of biofuel.

Features and Benefits

  • Aligns with AP Biology Big Ideas 2 and 4
  • Introduction to enzyme kinetics with real world application to biofuel energy
  • Enables both qualitative and quantitative measurements of reactions
  • Contains no caustic reagents

Classroom Experience

Students first extract cellobiase from mushrooms to discover one way that nature uses enzymes. They then generate questions and hypotheses about enzyme function under different environmental conditions — temperature, pH, and concentrations — that influence enzyme function. Students can take a structured, guided, or open approach to inquiry as they test their hypotheses. Finally, with data in hand, students use evidence to create a list of conditions for optimal enzymatic activity for biofuel production.

Timeline and Contents Overview

The flexible curriculum will fit within a broad range of available laboratory times. A possible arrangement is:

  • Day 1: Pre-lab activity and background review
  • Day 2: Investigation 1
  • Day 3: Investigations 2–5 (divided into groups)
  • Day 4: Investigation 6 and post-lab assessment
  • Day 5: Science Case Study

Background Information

Cellulosic ethanol is a biofuel derived from plant matter. Cellulose is a polysaccharide found in the cell walls of plants; and cellobiose is a disaccharide constituent of cellulose. The breakdown of cellulose into smaller sugars is a multistep process that is facilitated by a family of enzymes called cellulases. Each cellulase has its own role in processing cellulose from a long strand of glucoses down to single units that can then undergo microbial fermentation to produce ethanol.

A key step in the production of cellulosic ethanol is the breakdown of cellobiose by the cellobiase enzyme. Students test and calculate the conversion rate of a sugar substrate (p–nitrophenyl glucopyranoside) to p–nitrophenol and glucose in the presence or absence of cellobiase.

Select the kit which is right for you and your students:

 

ThINQ! Biofuel Kit
(17001235EDU)

Classic Biofuel Kit
(1665035EDU)

Standards alignment

AP Biology

General Biology, Biotechnology, CTE

Pedagogical support

Open, guided, and structured inquiry

Structured inquiry

Main learning objectives

Focus on student-driven questions and problem solving

Mastery of technical skills

Initial experiment

Compare reaction rates of mushroom extracts

Focuses on inquiry process

Compare reaction rates with and without purified enzyme

Uses well characterized samples to obtain reproducible results

Kit contents

Materials for 8 student workstations to perform 1 initial experiment and up to 5 additional enrichment activities

Explore features of the Classic Biofuel Enzyme Kit.

Specifications

Specifications

Kit contains sufficient materials for 8 student workstations (2–4 students per workstation)
 
Enzyme, cellobiase, 1 ml
1 vial
Substrate, p-nitrophenyl glucopyranoside, 90 mg
1 vial
Standard, p-nitrophenol, 1 mM, 4 ml
1 bottle
2x stop solution, 100 ml
1 bottle
10x resuspension buffer, 50 ml
1 bottle
Extraction buffer, 50 ml
1 bottle
Disposable plastic transfer pipets (DPTPs)
40
1.5 ml microcentrifuge tubes
90
15 ml conical tubes
50
1.5 ml standard disposable polystyrene cuvettes
100
Curriculum, including Teacher's Guide and Student Manual
1
Student Instruction Manual, available for download

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items

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166-5035EDU_tn.jpg
17001235EDU
Enzyme kinetics kit, includes enzyme, substrate, standard, solution, buffers, disposable pipets, tubes, and cuvettes for 32 students; inquiry-driven curriculum; education use only

Refills

Refills

1665036EDU
Consumables refill reagents for use with Biofuel Enzyme kit (1665035EDU) and Biofuel Enzyme Reactions Kit (17001235EDU), contains enzyme, substrate, standard, 2x stop solution, 10x resuspension buffer, extraction buffer; education use only.

Documents

Documents

Number Description Options
10000058537
Biofuel Enzyme Reactions Kit for AP Biology Student Manual, Rev A
6840
Biofuel Enzyme Reactions Kit for AP Biology Teacher Manual Preview,Ver A
10000059026
Biofuel Enzyme Reactions Kit for AP Biology Teacher Model Process,Rev A
6815
AP Biology Alignment, ThINQ!™ Biofuel Enzyme Reactions Kit for AP Biology
6845
Biofuel Enzyme Reactions Kit for AP Biology Planning Guide
6849
Biofuel Enzyme Reactions Kit for AP Biology Quick Guide, Ver A
6730
Experimental Design and Planning Worksheet
6696
Mushroom Habitat Flashcards, Rev A
10000058536
Biofuel Enzyme Reactions Kit Science Case Study, Ver A
6605
Map of AP-Aligned Bio-Rad Kits with Learning Objectives
7151
Science, Food, and Environment AP Strand Poster, Ver A