2-D Electrophoresis Buffers and Reagents

Overview

Buffers and reagents are offered for each step of the 2-DE separation process. The ReadyPrep 2-D starter kit is designed to simplify setting up the PROTEAN i12 IEF system and optimizing your 2-D electrophoresis technique.


Category Products

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    ReadyPrep™ 2-D Starter Kit
    The ReadyPrep 2-D starter kit is intended for first-time users of the PROTEAN IEF cell and ReadyStrip IPG strips. The kit contains tested premixed reagents and a reference manual with technical tips.
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    E. coli Lysate Sample
    This E. coli lysate sample is performance-tested to generate a consistent pattern when used with ReadyPrep 2-D starter kit rehydration/sample buffer. Use as a control to validate your 2-D protocol.
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    Overlay Agarose
    Overlay agarose saves time in setting up second-dimension SDS-PAGE samples. Bromophenol blue tracking dye is incorporated into these solutions to allow monitoring of electrophoresis runs.
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    Detergent for IEF
    Detergents are used in electrophoresis when it is necessary to disrupt protein-lipid or protein-protein interactions. SDS, Tween 20, Triton X-100 and CHAPS solutions are available.
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    Reducing and Alkylating Agents
    Reducing agents are commonly used during sample preparation to cleave disulfide bond crosslinks within proteins and between protein subunits. DTT, TBP and iodoacetamide are offered.
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    Ampholytes
    Bio-Lyte carrier ampholytes, supplied as clear aqueous solutions, are blended to give a complete range of isoelectric points for reproducible, linear pH gradients.