My Account

    USA

      0 Items
      Browse Catalog

      2-D Electrophoresis Buffers and Reagents

      readyprep 2-d starter kit

      Overview

      Buffers and reagents are offered for each step of the 2-DE separation process. The ReadyPrep 2-D starter kit is designed to simplify setting up the PROTEAN i12 IEF system and optimizing your 2-D electrophoresis technique.

      Category Products

      • lsr_readyprep2d_starter_kit_pdtn.jpg
        ReadyPrep™ 2-D Starter Kit
        The ReadyPrep 2-D starter kit is intended for first-time users of the PROTEAN IEF cell and ReadyStrip IPG strips. The kit contains tested premixed reagents and a reference manual with technical tips.
      • lsr_control_sample_pdtn.jpg
        E. coli Lysate Sample
        This E. coli lysate sample is performance-tested to generate a consistent pattern when used with ReadyPrep 2-D starter kit rehydration/sample buffer. Use as a control to validate your 2-D protocol.
      • lsr_overlay_agarose_pdtn.jpg
        Overlay Agarose
        Overlay agarose saves time in setting up second-dimension SDS-PAGE samples. Bromophenol blue tracking dye is incorporated into these solutions to allow monitoring of electrophoresis runs.
      • lsr_detergent_for_ief_pdtn.jpg
        Detergent for IEF
        Detergents are used in electrophoresis when it is necessary to disrupt protein-lipid or protein-protein interactions. SDS, Tween 20, Triton X-100 and CHAPS solutions are available.
      • lsr_reducing_alkylating_readyprep_pdtn.jpg
        Reducing and Alkylating Agents
        Reducing agents are commonly used during sample preparation to cleave disulfide bond crosslinks within proteins and between protein subunits. DTT, TBP and iodoacetamide are offered.
      • lsr_ampholytes_pdtn.jpg
        Ampholytes
        Bio-Lyte carrier ampholytes, supplied as clear aqueous solutions, are blended to give a complete range of isoelectric points for reproducible, linear pH gradients.