Detergent for IEF

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Overview

Detergents are used in electrophoresis when it is necessary to disrupt protein-lipid or protein-protein interactions. SDS, Tween 20, Triton X-100 and CHAPS solutions are available.
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Description

Description

Detergents are used in IEF to disrupt hydrophobic interactions and increase solubility of proteins at their pI. Detergents must be nonionic or zwitterionic to allow proteins to migrate according to their own charges. Some proteins, especially membrane proteins, require detergents for solubilization during isolation and to maintain solubility during focusing. Nonionic detergents such as octylglucoside and zwitterionic detergents such as CHAPS and its hydroxyl analog CHAPSO can be used for IEF applications.

Ionic detergents such as SDS are not compatible with IEF but can be used with concentrated samples in situations where the SDS can be unbound from the protein by IEF-compatible detergents that compete for binding sites.

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161-0460_tn.jpg
(Discontinued) CHAPS
1610460
1 g, CHAPS detergent powder

Documents

Documents

Number Description Options
3095
2-D Electrophoresis: Tools for Rapid, High-Resolution Protein Separations Brochure, Rev B
2651
2-D Electrophoresis Workflow How-To Guide, Rev F