Single-cell ATAC-seq

ATAC-seq

Explore the Epigenomic Landscape of Single Cells with the ATAC you seq

Gain access to high-performance chromatin profiling to unravel the complexities of cellular heterogeneity, differentiation, and disease-specific alterations in gene regulation. With ATAC-seq (Assay for Transposase-Accessible Chromatin using sequencing), you can delve into the regulatory landscape of individual cells, unlocking invaluable insights into the epigenomic mechanisms that shape their behavior.

  • Bio-Rad's Surecell ATAC-seq Library Prep Kit, coupled with the ddSEQ™ Single Cell Isolator, provides a simple and cost-efficient solution, making single-cell ATAC-seq more accessible than ever before. Generate high-resolution genome-wide data and achieve sensitive profiling of chromatin accessibility.

    Watch the video to learn more on the power and potential of single-cell ATAC-seq.

Discover simple, highly efficient droplet-based single-cell ATAC-seq

Experience the streamlined workflow, driven by our trusted digital droplet technology of ddSEQ Single-Cell Isolator and SureCell ATAC-seq Library Prep Kits.

Single-Cell ATAC-Sequencing applies to Steps 1 - 7
Omnition Analysis Software applies to Steps 8 - 9
Step 1
Cell Dissociation
and Lysis
Step 2
Single Nuclei
or Single Cells
Step 3
Tagmentation
Step 4
ddSEQ Single-Cell Isolator
Partitioning
Step 5
Droplet
PCR
Step 6
Bulk
PCR
Step 7
Sequencing
 
Step 8
Data Anaysis
Step 9
Report and Output Files

Imagine incorporating robust whole genome open chromatin interrogation into your current cell and translational studies. Get the efficiency, sample flexibility, scalability, and sensitivity of larger systems, but with affordability to do more with your budget.

What can you expect from ddSEQ and SureCell ATAC-seq?
Check out these features:

  • High cell capture efficiency (60-80%)
  • Flexible sample input: isolated nuclei or whole cells
  • Output scalability (400-5,000 single-cell ATAC-seq libraries per sample)
  • Fast; 5-minute partitioning and barcoding
  • Exceptional sensitivity (TSS Enrichment Scores>30, FRiP>30%)
  • Includes streamlined and robust Omnition Bioinformatics Analysis Software

Explore the Key Applications of Single-Cell ATAC-seq

Single-Cell Signature graphic

  • Cell type identification and heterogeneity

    Analyze chromatin accessibility profiles to determine distinct cell types, explore cellular heterogeneity, and identify rare cell populations.

  • Gene regulation and enhancer activity

    Investigate the regulatory landscape of individual cells, identify active enhancer regions, and unravel interactions between chromatin and gene-regulatory elements to understand gene expression regulation.

  • Cellular differentiation and development

    Study how cells differentiate into different cell types, uncover epigenetic mechanisms driving cellular differentiation, and gain insights into cell fate determination.

  • Disease mechanisms and pathology

    Explore disease-specific alterations in chromatin accessibility, identify regulatory elements associated with diseases, and understand the underlying epigenetic mechanisms contributing to disease progression.

  • Integration with other -omics data

    Combine single-cell ATAC-seq with other techniques, such as single-cell RNA sequencing (scRNA-seq), for multi-omic analyses, enabling a deeper understanding of gene regulation in cellular processes and disease.

Attend Our Latest Single-Cell ATAC-seq Events

  • American Society of Human Genetics
    ASHG 2023, CoLab Presentation

    Studying tumor heterogeneity using single cell ATAC-seq

    Speaker: Fatima Valdes-Mora, PhD, Team Leader, Cancer Epigenetic Biology and Therapeutics, Children's Cancer Institute, UNSW Australia and Xiaoti Guo, Sr Product Manager, Single Cell Technologies, Bio-Rad Technologies
    Date & Time: Thursday, November 2, 2023 from 1:05-1:35 pm EST

    Learn More

  • Fatima Valdes Mora PHD
    Webinar

    Deciphering Cancer Cell Heterogeneity for Precision Medicine

    Speakers: Fatima Valdes-Mora, PhD, Team Leader, Cancer Epigenetic Biology and Therapeutics, Children's Cancer Institute, UNSW Australia
    Date & Time: On-Demand 24/7

    Watch Now

 

Browse Our Resources for Single-Cell ATAC-seq

Product Information

  • ddseq-single-cell-isolator

    ddSEQ Cell Isolator

    Explore the ddSEQ Single-Cell Isolator, a droplet-based single cell isolation system, for single-cell chromatin accessibility studies. The system includes the isolator, test cartridges, and a cartridge holder.

  • surecell-atac-seq-library-prep-kit

    SureCell ATAC-Seq Library Prep Kit

    Understand gene regulation at an unprecedented scale with single-cell precision using the SureCell ATAC-Seq Library Prep kit, a highly sensitive, single-cel ATAC-Seq assay for genome-wide open chromatin interrogation.

  • omnition-analysis-software

    Omnition Analysis Software

    Utilize a robust pipeline analysis tool for processing, visualizing, and reporting single-cell ATAC-Seq data. The Omnition Analysis Software, offers better usability and functionality when compared other analysis tools.

  • ATAC-seq index kit

    SureCell ATAC-Seq Index Kit

    Simplify single-cell sequencing with the SureCell ATAC-Seq Index Kit, which contains indexed primer pairs for use with the ddSEQ Single-Cell Isolator and the SureCell ATAC-Seq Library Prep Kit.

ATAC you seq

Want to chat about scATAC-seq for your research with a Bio-Rad Single Cell expert? Contact us below.

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