ddSEQ Isolator for Single-Cell Sequencing

ddSEQ Isolator for Single-Cell Sequencing

Overview

ddSEQ Single-Cell Isolator is a droplet-based single cell isolation system for single-cell gene expression and gene regulation studies. The system includes the isolator, test cartridges and the cartridge holder

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Description

Description

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The ddSEQ Single-Cell Isolator is part of the Bio-Rad Single-Cell Next-Generation Sequencing (NGS) Solution, which works with the Bio-Rad Library Preparation Kits for single-cell 3’ RNA-Seq and ATAC-Seq. The technology utilizes disposable microfluidic cartridges to coencapsulate single cells or single nuclei and barcoded beads into subnanoliter droplets, where cell-bead pairing and barcoding occur. 3’ RNA-Seq and ATAC-Seq libraries are subsequently prepared and sequenced, and analysis is conducted with the Bio-Rad Omnition Analysis Software.

Features and Benefits

  • Process hundreds to tens of thousands of cells per experiment
  • Sensitive and unbiased characterization of gene expression and gene regulation
  • Scalable solution to accommodate a wide range of experimental designs
  • Agnostic to mammalian cell size, enabling unbiased profiling of diverse cell populations
  • End-to-end single-cell sequencing solution with simple, powerful data analysis

Applications and Uses

Gain insights into cellular heterogeneity, empowering a wide range of fields including, but not limited to:

  • Cancer research — cellular expansion of clonal cells in tumors and mutations that lead to differences in gene expression and drug response
  • Immunology research — B Cell and T Cell characteristic and functional analyses
  • Neurology research — normal brain function, neurological disease and psychological disorders
  • Stem cell research — cell fate, cell development and differentiation, including reverse engineering tissues

Included Components

  • ddSEQ Test Cartridges (12003862)
  • ddSEQ Cartridge Holder (12004739)
  • ddSEQ Single-Cell Isolator (12004336)
  • Power supply and power cord
  • User manual and product documentation

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12004336

ddSEQ Single-Cell Isolator is a droplet-based single cell isolation system for single-cell gene expression and gene regulation studies. The system includes the isolator, test cartridges and the cartridge holder

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Accessories

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12001925

Pkg of 25, clear well/clear shell semi-skirted plates, for use with QX600/QX200 Droplet Digital™ PCR Systems

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1450102

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1851197

Modular thermal cycler platform, includes C1000 Touch Thermal Cycler chassis, 96–deep well reaction module, USB flash drive

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