ddSEQ Isolator for Single Cell Sequencing

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Overview

The ddSEQ Single-Cell Isolator is a droplet-based single cell partitioning system for single-cell NGS workflow. The system includes the isolator, test cartridges and the cartridge holder.

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Description

Description

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The ddSEQ Single-Cell Isolator is part of the Bio-Rad Single-Cell Next-Generation Sequencing (NGS) Solution, a platform that brings Bio-Rad's Droplet Digital technology to single-cell gene expression and chromatin accessibility studies.

The ddSEQ Single-Cell Isolator works with the Bio-Rad SureCell ATAC-Seq and Illumina Bio-Rad SureCell WTA 3' Library Prep Kits. The technology utilizes disposable microfluidic cartridges to coencapsulate single cells or single nuclei and barcoded beads into subnanoliter droplets, where cell-bead pairing and barcoding occur. ATAC-Seq or RNA-Seq libraries are subsequently prepared and sequenced, and analysis is conducted with the Bio-Rad ATAC-Seq analysis pipeline or via BaseSpace.

Features and Benefits

  • Process hundreds to tens of thousands of cells per experiment
  • Sensitive and unbiased characterization of chromatin accessibility or transcriptional signatures
  • Scalable solution to accommodate a wide range of experimental designs
  • Agnostic to mammalian cell size, enabling unbiased profiling of diverse cell populations
  • End-to-end single-cell sequencing solution with simple, powerful data analysis

Applications and Uses

  • Assess cell-to-cell heterogeneity and identify new sub-populations
  • Map genome-wide chromatin accessibility
  • Dissect transcriptional mechanics
  • Infer gene regulatory networks
  • Neurology research — normal brain function, neurological disease and psychological disorders
  • Immunology research — B Cell and T Cell characteristic and functional analyses
  • Cancer research — cellular expansion of clonal cells in tumors and mutations that lead to differences in gene expression and drug response
  • Stem cell research — cell fate, cell development and differentiation, including reverse engineering tissues

Included Components

  • ddSEQ Test Cartridges (12003862)
  • ddSEQ Cartridge Holder (12004739)
  • ddSEQ Single-Cell Isolator (12004336)
  • Power supply and power cord
  • User manual and product documentation

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12004336

ddSEQ Single-Cell Isolator is a droplet-based single cell isolation system for single-cell chromatin accessibility and gene expression studies. The system includes the isolator, test cartridges and the cartridge holder

Accessories

Accessories

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12001925

Pkg of 25, clear well/clear shell semi-skirted plates, for use with QX200™/QX100™ or QX200™ AutoDG™ Droplet Digital™ PCR Systems

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1450102
120–240 V, includes instrument, power supply, USB flash drive, 30 dual-chamber counting slides (60 counts), 1.5 ml trypan blue
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1851197

Modular thermal cycler platform, includes C1000 Touch Thermal Cycler chassis, 96–deep well reaction module, USB flash drive

Documents

Documents

Number Description Options
7168
Droplet Digital Sequencing (ddSEQ) Solution Publications List
7055
Identifying Heterogeneity within Rare Cell Populations by Pairing Single-Cell RNA-Seq with Cell Sorting
6855
Illumina Bio-Rad Single-Cell Sequencing Solution Brochure
10000069430
ddSEQ Single-Cell Isolator Instruction Manual
6863
Introducing the ddSEQ Single-Cell Isolator, SureCell WTA 3' Library Prep Kit, and SeqGeq Analysis Software Flier
7139
Implementing the Drop-Seq Protocol on Bio-Rad’s ddSEQ Single-Cell Isolator