Description
Description
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The ddSEQ Single-Cell Isolator is part of the Bio-Rad Single-Cell Next-Generation Sequencing (NGS) Solution, which works with the Bio-Rad Library Preparation Kits for single-cell 3’ RNA-Seq and ATAC-Seq. The technology utilizes disposable microfluidic cartridges to coencapsulate single cells or single nuclei and barcoded beads into subnanoliter droplets, where cell-bead pairing and barcoding occur. 3’ RNA-Seq and ATAC-Seq libraries are subsequently prepared and sequenced, and analysis is conducted with the Bio-Rad Omnition Analysis Software.
Features and Benefits
- Process hundreds to tens of thousands of cells per experiment
- Sensitive and unbiased characterization of gene expression and gene regulation
- Scalable solution to accommodate a wide range of experimental designs
- Agnostic to mammalian cell size, enabling unbiased profiling of diverse cell populations
- End-to-end single-cell sequencing solution with simple, powerful data analysis
Applications and Uses
Gain insights into cellular heterogeneity, empowering a wide range of fields including, but not limited to:
- Cancer research — cellular expansion of clonal cells in tumors and mutations that lead to differences in gene expression and drug response
- Immunology research — B Cell and T Cell characteristic and functional analyses
- Neurology research — normal brain function, neurological disease and psychological disorders
- Stem cell research — cell fate, cell development and differentiation, including reverse engineering tissues
Included Components
- ddSEQ Test Cartridges (12003862)
- ddSEQ Cartridge Holder (12004739)
- ddSEQ Single-Cell Isolator (12004336)
- Power supply and power cord
- User manual and product documentation
Related Products
- ddSEQ Single-Cell 3' RNA-Seq Kit
- ddSEQ Single-Cell ATAC-Seq Kit
- 96-Well Plate Cooling Block
- QX200 AutoDG Droplet Digital PCR System
- TC20 Cell Counter
- C1000 Touch Thermal Cycler
- ddPCR 96-Well Plates
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ddSEQ Single-Cell Isolator is a droplet-based single cell isolation system for single-cell gene expression and gene regulation studies. The system includes the isolator, test cartridges and the cartridge holder
Accessories
Accessories
Pkg of 1, 96-Well Plate Cooling Block for use with the ddSEQ™ Single-Cell Isolator
Pkg of 1, replacement cartridge holder for the ddSEQ Single-Cell Isolator
Pkg of 2, replacement test cartridges for use with the ddSEQ Single-Cell Isolator
Pkg of 25, clear well/clear shell semi-skirted plates, for use with QX600/QX200 Droplet Digital™ PCR Systems
120–240 V, includes instrument, power supply, USB flash drive, 30 dual-chamber counting slides (60 counts), 1.5 ml trypan blue
Modular thermal cycler platform, includes C1000 Touch Thermal Cycler chassis, 96–deep well reaction module, USB flash drive