Description
Description
The ESR1 gene encodes estrogen receptor (ER) subtype alpha. The ddPLEX ESR1 Mutation Detection Kit quantitatively interrogates seven key ESR1 variants across three exons: exon 5 (E380Q), exon 7 (S463P) and exon 8 (L536R, Y537C, Y537S, Y537N and D538G), and their wild-type counterparts.
The kit consists of sufficient reagents for 120 reactions (including 60 reactions of each for the Mutant Assay and Wild-Type Assay), a synthetic DNA based ESR1 Positive Control, multiplex supermix, and DTT.
The kit is used with the Bio-Rad QX600™ Droplet Digital™ PCR System or QX600™ AutoDG Droplet Digital™ PCR System and is compatible with the QX Manager Standard Edition and Premium Edition Software for data analysis.
Features & Benefits
- Highly sensitive (0.01%–0.025% VAF*) single-well discrimination of seven key ESR1 gene mutations
- Same-day results with a streamlined workflow
- Flexible sample input of DNA from plasma or FFPE
- Kit includes positive and internal controls
- Sensitivity increased with patented wild-type suppression chemistry
- ddPCR-powered reproducibility and specificity using the QX600 ddPCR system and QX Manager Software
* Variant Allele Frequency
Applications & Uses
- For the detection, discrimination and quantification of key ESR1 mutations for breast cancer research, from cfDNA in plasma and/or DNA in formalin-fixed paraffin embedded (FFPE) tissue samples.
Product Contents
Included Components
The kit includes the following components for 120 reactions:
| Material Number | Description | Quantity |
|---|---|---|
| 12019648 | ddPLEX ESR1 Mutation Detection Assay ddPLEX ESR1 Mutant Assay (85 µl, 60 reactions) ddPLEX ESR1 Wild-Type Assay (85 µl, 60 reactions) ddPLEX Enhancer (100 µl) ddPLEX Internal Control (500 µl) |
1 |
| 12019606 | ddPLEX ESR1 Positive Control, 100 µl | 1 |
| 12005909 | ddPCR Multiplex Supermix, 1.2 ml | 1 |
| 12012171 | Dithiothreitol (DTT), 2 ml | 1 |
The four components listed above can also be purchased individually.
Configuration Options
The ddPLEX ESR1 Mutation Detection Assay should be used with the QX600 ddPCR System and either the Manual Droplet Generator or the Automated Droplet Generator. Equipment, key consumables, and reagents necessary for the QX600 System are listed below (additional items may be needed, please see the User Guide for more details)
Key Droplet Generation & Reader Equipment, Consumables & Reagents
| Description | Catalog Number |
|---|---|
| QX600 Droplet Reader | 12013328 |
| Automated Droplet Generator | 1864101 |
| QX200 Manual Droplet Generator | 1864002 |
| PTC Tempo Thermal Cycler | 12015392 |
| PCR Plate Heat Seal | 1814040 |
| PX1 PCR Plate Sealer | 1814000 |
| ddPCR Plates 96-well Semi-skirted | 12001925 |
| ddPCR Droplet Reader Oil | 1863004 |
| ddPCR Buffer Control for Probes (2X) | 1863052 |
Reagents & Consumables for Automated Droplet Generation Only
| SKU Number | Product Name |
|---|---|
| 1864110 | Automated Droplet Generator Oil for Probes |
| 1864108 | DG32 Automated Droplet Generator Cartridges |
| 1864120 | Pipet Tips for Automated Droplet Generator |
| 1864125 | Pipet Tip Bins for Automated Droplet Generator |
Reagents & Consumables for Manual Droplet Generation Only
| SKU Number | Product Name |
|---|---|
| 1863005 | Droplet Generator Oil for Probes |
| 1863051 | Droplet Generator DG8 Cartridge Holder |
| 1864008 | DG8 Cartridges for QX100/QX200 Droplet Generator |
| 1863009 | DG8 Gaskets for QX100/QX200 Droplet Generator |
Mutations Detected by ddPCR Multiplex Mutation Screening Kits
| Catalog # | ddPCR Multiplex Mutation Screening Kit* | Mutations Detected |
|---|---|---|
| 12015172 | MSI** | BAT25/26, NR21/24, Mono27 |
| 12001037 | BRAF V600 | V600E, V600K, V600R |
| 1863506 | KRAS G12/G13 | G12A, G12C, G12D, G12R, G12S, G12V, G13D |
| 12001626 | KRAS Q61 | Q61H (183A>C), Q61H (183A>T), Q61K, Q61L, Q61R |
| 12001094 | NRAS G12 | G12A, G12C, G12D, G12R, G12S, G12V |
| 12001627 | NRAS G12/G13 | G12A, G12C, G12D, G12S, G12V, G13D, G13R, G13V |
| 12001006 | NRAS Q61 | Q61H (183A>C), Q61H (183A>T), Q61K, Q61L, Q61R |
| 12002392 | EGFR exon19 deletions | >15 EGFR exon19 deletions |
*Assays for each of the mutations detected by these kits can also be ordered individually. Each kit contains 20x ddPCR Mutation Screening Multiplex Assay (1 x 200 μl) and 2x ddPCR Supermix for Probes (No dUTP) (2 x 1 ml).
**Each kit contains ddPCR Multiplex Supermix (2x 600 ul), ddPCR MSI Assay 1 (BAT25/26), ddPCR MSI Assay 2 ((NR21/24), ddPCR Assay 3 (Mono27), Uracil-DNA Glycosylase (UDG), positive control, and nuclease-free water.
Software Compatibility
The ddPLEX ESR1 Mutation Detection Kit is compatible with the following QX Software selections:
| SKU Number | Product Name |
|---|---|
| (Free download) | QX Manager Software, Standard Edition |
| 12018108 | QX Manager Software, Premium Edition |
Related Products
- ddPCR Microsatellite Instability (MSI) Kit
- BRAF V600 ddPCR Multiplex Mutation Screening Kit
- KRAS ddPCR Multiplex Mutation Screening Kits
- NRAS ddPCR Multiplex Mutation Screening Kits
- EGFR Exon19 Deletions ddPCR Multiplex Mutation Kit
Related Category
Supporting Documents
For Research Use Only. Not for use in diagnostic procedures.