ROX Passive Reference Dye #1725858


0.5 ml, passive reference dye for use on ROX-dependent real-time PCR instruments

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172-5858-MSDS ROX Passive Reference Dye English

Many real-time thermal cyclers require an internal reference dye for fluorescent signal normalization and correction of well-to-well optical variations. ROX passive reference dye allows seamless integration of non-ROX-containing PCR reagents on all ROX-dependent real-time instrument systems. Since excitation optics of different instrument platforms vary, the optimal reference dye concentration must be matched to the given real-time cycler. This dye has been formulated as a 50x concentrated stock solution for use on the ABI PRISM 7000, 7300, 7700, or 7900 systems. For instruments that use 580–585 nm excitation for passive reference, such as the Stratagene Mx4000, Mx3000P, or Mx3005P, or the ABI PRISM 7500 sequence detection system, the ROX passive reference dye should be treated as a 750x concentrated solution. An internal reference is not required for use with the iCycler iQ®, MyiQ™, DNA Engine® Opticon 2, or Chromo4™ real-time detection systems.