DEAE Affi-Gel Blue Gel is a bifunctional affinity/ion exchange chromatography matrix prepared by coupling Cibacron Blue F3GA and diethylaminoethyl groups to Bio-Gel A Gel. The Cibacron blue F3GA functions as an ionic, hydrophobic, or sterically active binding site for proteins with dinucleotide folds, such as albumin. The diethylaminoethyl functional group acts as an anion exchanger and binds proteins with isoelectric points higher than the pH of the mobile phase. This bifunctionality allows you to obtain highly purified IgG from serum from a wide variety of species through careful optimization of the ionic strength and pH of the application buffer.
Features and Benefits of DEAE Affi-Gel Blue Gel
- Single-step IgG purification from serum; the eluted IgG contains a small amount of transferrin (samples are diluted approximately 5-fold)
- Albumin removal
- No detectable proteolytic activity in the eluted IgG fraction
- Economical alternative to protein A affinity chromatography
Instruction Manual, DEAE Affi-Gel® Blue Support (Bulletin 9191)