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Droplet Digital PCR is an elegant technology that permits accurate and absolute quantification of target nucleic acid molecules. By partitioning a PCR reaction into thousands of nanoliter-sized droplets followed by amplification and subsequent interrogation of each droplet, Droplet Digital PCR (ddPCR) assays allow increased quantitative resolution, enhanced detection of difficult-to-amplify targets, better reproducibility, and simplified data analysis. This webinar provides an overview of ddPCR technology from technical and workflow-related perspectives and reviews key applications such as copy number variation (CNV) analysis, rare mutation detection (RMD), and standard-free (stand-alone) absolute quantification of nucleic acids.