Abstract:
This webinar is for 2-D novices and experts alike who want to hone their skills and keep abreast of new advances in the field. Dr. Anton Posch, a renowned expert in 1- and 2-D electrophoresis, provides insights on sample preparation, isoelectric focusing (IEF), SDS-PAGE, and image analysis for 2-D electrophoresis. Included are techniques to improve reproducibility and minimize experimental error. He also shows how to troubleshoot issues with your 2-D protocol.
Part I presents the main steps of the 2DE workflow, starting with sample preparation including protein extraction and solubilization, irreversible reduction and alkylation of protein side chains, and critical success factors for IEF.
Also see 2-D Electrophoresis Tips & Tricks — Part II for tips on IEF separation using immobilized pH gradient (IPG) strips, running second-dimension PAGE, spot detection, gel matching, and data analysis, followed by Q&A on topics including sample preparation, sample storage, and running conditions
About the speaker:
Dr. Anton Posch has provided hands-on training in this field for 19 years and has more than 30 peer-reviewed publications. He currently sits on the editorial boards of both Proteomics and Proteomics — Clinical Applications and is the editor of "2-D PAGE: Sample Preparation and Fractionation," among the top 25% books downloaded in the 2012 Springer eBook collection.