Chromatographic analysis of plasma is an extremely useful diagnostic tool. In this section, we present an example of how chromatography was used to analyze disease profiles.
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An increase in serum lactate and pyruvate levels is associated with several disorders. High serum lactate levels can indicate lactic acidosis in diabetes, and can be a useful prognostic indication in myocardial infarction complicated by shock. A rise in serum pyruvate levels can indicate heavy metal poisoning or thiamine deficiency. The Aminex HPX-87H column can solve many of the analytical problems associated with the determination of serum lactate and pyruvate. The figure below shows lactate, pyruvate, and urate levels in normal plasma, alongside six additional plasma samples indicative of various diseases. As the chromatograms clearly demonstrate, the Aminex HPX-87H column provides the most convenient method for analysis of acids in biological samples.
Plasma profiles generated using Aminex HPX-87H column. A, normal, 0.7 ml plasma; B, methylmalonic acidemia, 0.5 ml plasma; C, propionic acidemia 0.7 ml plasma; D, maple syrup urine disease, 0.4 ml plasma; E, severe ketosis, 0.1 ml plasma; F, isovaleric acidemia, 0.5 ml plasma; G, 3-hydroxy-3-methylglutaryl-CoA lyase deficiency, 0.4 ml plasma.
Bio-Rad (1999). Guide to Aminex HPLC columns for food and beverage, biotechnology, and bio-organic analysis. Bio-Rad Bulletin 1928.
Daish P and Leonard JV (1985). Rapid profiling of plasma organic acids by high performance liquid chromatography. Clinica Chimica Acta 146, 87–91.
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