1 X 96-well Flat Bottom Plate, x-Plex assay format that includes premixed coupled magnetic beads, premixed detection antibodies, lysates, and reagents for detecting phosphorylated Akt (Ser473), Erk1/2 (Thr202/Tyr204, Thr185/Tyr187), GSK-3α/β (Ser21/Ser9), JNK (Thr183/Tyr185), NF-κB p65 (Ser536), all in one kit.