1 X 96-well flat bottom plate, x-Plex assay format that includes premixed coupled magnetic beads, detection antibodies, standards, reagents, and diluents for detecting human cytokines IL-6, IL-8, IL-12 (p70), IFN-ƴ, MCP-1 (MCAF), MIP-1α, MIP-1ß, TNF-α, all in one kit.