RC DC™ Protein Assay Kit II #5000122

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Overview

Colorimetric assay kit, includes RC Reagents Package, DC™ Protein Assay Reagents Package, BSA standard; 450 standard assays

Safety Data Sheet

Safety Data Sheet

Description

Description

Use the RC DC Protein Assay for samples containing reducing agents and detergents. This assay, based on the DC Protein Assay, is a modified form of the Lowry assay that is both reducing agent compatible (RC) and detergent compatible (DC), whereas the DC Assay is only detergent compatible.

  • Linear over a wide range of protein concentration; standard assay, 0.125–1.5 mg/ml and low concentration assay, 5–250 µg/ml
  • Read wavelengths, 650–750 nm
  • Absorbance values are stable ≥ 1 h
  • Quantitate protein in sample loading buffer prior to gel electrophoresis, either 1-D or 2-D format
  • Determine protein recovery in cell lysates

Sample components compatible with the RC DC Protein Assay include:

  • Ammonium sulfate, 0.5 M
  • β-mercaptoethanol, 10%*
  • Brij 35, 1%
  • C12E8, 0.2%
  • Calcium chloride, 50 mM
  • CHAPS, 2%
  • CHAPSO 1%
  • Dithiothreitol (DTT), 350 mM*
  • EDTA 0.1 M
  • Guanidine hydrochloride, 0.4 M
  • Imidazole, 0.5 M
  • Laemmli buffer (5% β-mercaptoethanol)
  • Nonidet P-40, 2%
  • Octyl-β-glucoside, 1%
  • ReadyPrep™ Sequential Extraction Reagents 2 and 3*
  • SDS, 10%
  • Tributylphosphine (TBP), 2 mM
  • Tris, pH 8.4, 0.5 M
  • Triton X-100, 2%
  • Tween 20, 2%
  • Urea, 4 M

 

 

*Two washes required for highest concentrations

 

 

Product Contents

  • RC Reagent I, 250 ml
  • RC Reagent II, 250 ml
  • Alkaline copper tartrate, 250 ml
  • Dilute Folin reagent, 2 L
  • Surfactant solution, 5 ml
  • Lyophilized bovine serum albumin (BSA), 1 vial

Packaging Options

Kit Protein Standard Catalog Number
RC DC Protein Assay Kit I Bovine γ-globulin 5000121
RC DC Protein Assay Kit II Bovine serum albumin 5000122

 

Related Products

 

  • Protein Standard I, lyophilized bovine γ-globulin (5000005)
  • Protein Standard II, lyophilized bovine serum albumin (5000007)
  • Semimicrovolume Disposable Polystyrene Cuvettes (2239955)
  • Standard Disposable Polystyrene Cuvettes (2239950)
  • Protein Assay Reagent A, refill, 250 ml alkaline copper tartrate (5000113)
  • Protein Assay Reagent B, refill, 1 L Folin reagent (5000114)
  • Protein Assay Reagent S, refill, 5 ml surfactant (5000115)
  • RC Reagent I, refill, 250 ml (5000117)
  • RC Reagent II, refill, 250 ml (5000118)

Related Categories

Supporting Documentation

Reference

Lowry OH et al. (1951). Protein Measurement with the Folin Phenol Reagent. J Biol Chem 193, 265-275. PMID: 14907713