SingleShot™ Probes Kit for Cell Lysis and RT-qPCR, 100 x 50 µl reactions #1725090

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Overview

Pkg of 1, kit includes SingleShot Cell Lysis Reagents, iScript™ Advanced cDNA Synthesis Kit for RT-qPCR, SsoAdvanced™ Universal Probes Supermix, and the SingleShot RNA Control, for 100 x 50 µl reactions
Safety Data Sheet

Safety Data Sheet

Description

Description

Use the SingleShot Probes Kit to obtain high-quality RT-qPCR data with probe chemistry directly from cell culture lysates.

Two-step workflow provides results in less than 2 hours after cell lysis. All RT-qPCR reagents and RNA control included.

Benefits include:

  • Cell lysate from 10 to 100,000 cells ready in 20 minutes
  • Complete removal of genomic DNA while preserving RNA integrity
  • Optimal accuracy and linear dynamic range of qPCR data across genes
  • Results comparable to using purified RNA, including dynamic range
  • Validated with PrimePCR™ Assays and Panels (visit www.bio-rad.com/primepcr)
  • SingleShot RNA Control ensures optimal cell and lysate inputs

Optimized Sample Input

The SingleShot Probes Kit maximizes cell lysate and cDNA inputs in a two-step RT-qPCR reaction:

Maximum cell lysate into RT reaction 45% of total volume
Maximum undiluted cDNA into qPCR reaction 20% of total volume

Kit Contents

SingleShot Cell Lysis Reagents
SingleShot Cell Lysis Buffer
SingleShot Proteinase K Solution
SingleShot DNase Solution

100 x 50 μl reactions
5 ml (1 x 5 ml vial)
100 μl (1 x 1 ml vial)
100 μl (1 x 1 ml vial)

cDNA Synthesis Kit
5x iScript Advanced Reaction Mix
iScript Advanced Reverse Transcriptase
Nuclease-Free Water

100 reactions
400 μl (1 x 1 ml vial)
100 μl (1 x 1 ml vial)
1 ml (1 x 1 ml vial)

qPCR Supermix
SsoAdvanced Universal Probes Supermix

500 reactions
5 ml (5 x 1 ml vials)

SingleShot RNA Control
SingleShot RNA Control Template
SingleShot Probes qPCR Control Assay

200 reactions
Lyophilized (200 reactions)
200 μl (1 x 1 ml vial)

Refer to the user manual for details on reaction setup and troubleshooting tips.

Cell Lines Validated for Use with the SingleShot Probes Kit

The SingleShot Probes Kit has been validated for use with a number of common human and rodent cell lines, such as all types of adherent and suspension cells, including but not limited to:

Cell Line Property Species
A549 Adherent  H. sapiens, lung carcinoma
HeLa Adherent H. sapiens, cervical adenocarcinoma
HepG2   Adherent  H. sapiens, liver carcinoma
NIH/3T3 Adherent M. musculus, embryonic fibroblast
MCF-7 Adherent  H. sapiens, epithelial, adenocarcinoma
NCI-H1975  Adherent H. sapiens, lung adenocarcinoma 
K-562 Suspension H. sapiens, chronic myelogenous leukemia
HEK 293 Suspension H. sapiens, kidney
Jurkat Suspension H. sapiens, T lymphocyte
PC-12  Suspension R. norvegicus, pheochromocytoma, adrenal
HepRG   Primary-like H. sapiens, hepatocytes
 

Related Products

The SingleShot Probes Kit belongs to the family of SingleShot™ Cell Lysis RT-qPCR Kits:

  • SingleShot Cell Lysis One-Step RT-qPCR Kits — including SingleShot™ SYBR® Green One-Step Kit (172-5095) and SingleShot Probes One-Step Kit (172-5070)
  • SingleShot Cell Lysis RT-qPCR Kits — including SingleShot™ SYBR® Green Kit (172-5085) and SingleShot Probes Kit (172-5090)
  • SingleShot Cell Lysis Kit — available for 100 reactions (172-5080)

Specifications

Specifications

Storage at –20°C
12 months:
Lysis buffer, DNase, and proteinase K solution
iScript Advanced cDNA Synthesis Kit
SsoAdvanced Universal Probes Supermix
SingleShot RNA Control Template
SingleShot Probes qPCR Control Assay
Storage at 4°C
6 months:
Lysis buffer

Up to 3 months:
SsoAdvanced Universal Probes Supermix