Bio-Scale™ Mini Profinity™ IMAC Cartridges

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Overview

Bio-Scale Mini Profinity IMAC Cartridges are 1 ml and 5 ml Bio-Scale Mini Cartridges used for the purification of recombinant histidine (His)-tagged proteins.

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Description

Description

Bio-Scale Mini Profinity IMAC cartridges are 1 ml and 5 ml Bio-Scale Mini cartridges filled with Profinity Ni-charged IMAC resin. They are used for the purification of recombinant histidine (His)-tagged proteins by immobilized metal affinity chromatography (IMAC).

IMAC is a powerful purification technique that relies on the affinity that His-tag has for immobilized transition metals. Profinity IMAC resins, based on UNOsphere™ beads, contain iminodiacetic acid (IDA) as the chelating ligand for di- or trivalent metal ions. Its chemical structure allows highly selective binding of recombinant His-tagged proteins when charged with Ni2+ or other transition metals, such as Zn2+ or Cu2+. Profinity IMAC offers high capacity at high flow rates. It can be used under either nondenaturing or denaturing conditions.

Features of Profinity IMAC resins include:

  • Optimal ligand density for higher purity of target protein
  • Superb mechanical strength
  • Excellent pressure-flow properties, for high maximum operating pressures and high flow rates, which allow rapid purification as well as rapid column cleaning and reequilibration
  • Stable from pH 1 to 14
  • Compatible with denaturing agents, detergents, and reducing agents

Bio-Scale Mini cartridges have a double-wall cartridge design* that provides extra durability and allows easy, reliable runs at pressures up to 45 psi with the aqueous buffers most commonly used for protein separation. Convenient, disposable, and ready to use, these cartridges are designed for use with BioLogic™ systems, a peristaltic pump, or any chromatography system. Bio-Scale Mini affinity cartridges can also be run on the Profinia™ protein purification system. For simple step elution, the cartridges can be used with a Luer-Lok syringe. Fittings are available for connection to HPLC- and FPLC-type systems.

Other features include:

  • Simple luer fittings for easy connection to any system
  • Chemically compatible polypropylene parts
  • A wide range of chromatography media

More Information

High Performance Columns For Antibody and Tagged Protein PurificationsLearn more about Bio-Rad’s suite of high-performance chromatography columns designed for high-resolution laboratory scale protein purification.







Related Products

Fitting adaptors are available for connection to other low- and medium-pressure chromatography systems:

  • Fittings kit for connection to BioLogic DuoFlow™ systems (732-0113)
  • Fittings kit for connection to older AKTA systems with M6 fittings (732-0111)
  • Fittings kit for connection to NGC™ systems and newer AKTA systems with 1/16th inch/10-32 fittings (732-0112)

Bio-Rad chromatography systems:

 * U.S.patent 7,208,087.

Specifications

Specifications

Sizes
1 ml and 5 ml bed volumes; desalting cartridges: 10 ml and 50 ml bed volumes
Recommended flow rate
Varies for each support type*
Maximum operating pressure
1, 5, and 10 ml: 45 psi
50 ml: 40 psi
Fittings
Female luer inlet and male luer outlet
Column material
Polypropylene
Frit material
Polyethylene (HDPE)
Dimensions
1 ml: 40 mm long x 5.6 mm inner diameter
5 ml: 40 mm long x 12.6 mm inner diameter
10 ml: 80 mm long x 12.6 mm inner diameter
50 ml: 80 mm lomg x 28.2 mm inner diameter
* Recommended flow rates are given for each support type in the instruction manual.
 

Ordering

Ordering

items

Use the filters below to refine results!

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7324610
Pkg of 5, 1 ml, prepacked Ni-charged column, 40 mm x 5.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system
732-4612_tn.jpg
7324612
Pkg of 1, 5 ml, prepacked Ni-charged column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system
732-4614_tn.jpg
7324614
Pkg of 5, 5 ml, prepacked Ni-charged column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system
732-4620_tn.jpg
7324660

Pkg of 5, 1 ml, empty chromatography columns, 40 mm x 5.6 mm, max. pressure 45 psi, for use with BioLogic™ systems or any chromatography system

732-4624_tn.jpg
7324661
Pkg of 5, 5 ml, empty chromatography columns, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic™ systems or any chromatography system

Documents

Documents

Number Description Options
10005455
Instruction Manual, Bio-Scale™ Mini Profinity™ IMAC Cartridges, 1 and 5 ml, Rev D
6498
Lab-Scale Protein Purification – Column and Cartridge Selection Guide, Rev C
[ Add to Cart (Free) ]
5584
Bio-Scale Mini Cartridges, Product Information Sheet, Rev B
5514
Automated Purification of a His-Tagged Protein With the Profinia Protein Purification System: Comparison With Another Low-Pressure Chromatography System, Rev A
5574
Bio-Scale Mini Profinity Cartridges Product Information Sheet, Rev A
5444
Bio-Scale Mini Profinity IMAC Cartridge Product Information Sheet, Rev A
5864
Regeneration of Prepacked IMAC Cartridges on the Profinia™ Protein Purification System, Rev A