Bio-Scale™ Mini Profinity™ IMAC Cartridges

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Bio-Scale Mini Profinity IMAC Cartridges

Overview

Prepacked Chromatography Cartridge Selection Guide
Cartridge by Application Support Type of Separation Chemical Form Protein Capacity/ml* Catalog Numbers
by Cartridge Size
5 x 1 ml 1 x 5 ml 5 x 5 ml
Protein and Plasmid Purification
High Q Macro-Prep High Q Strong anion exchange –N+(CH3)3 ≥37 mg BSA 7324120 7324122 7324124
UNOsphere Q UNOsphere Q Strong anion exchange –N+(CH3)3 180 mg BSA 7324100 7324102 7324104
UNOsphere S UNOsphere S Strong cation exchange –SO3- 60 mg human lgG 7324110 7324112 7324114
DEAE Macro-Prep DEAE Weak anion exchange –HN+(C2H5)2 >35 mg protein 7324140 7324142 7324144
Protein Purification
High S Macro-Prep High S Strong cation exchange –SO3- ≥49 mg human lgG 7324130 7324132 7324134
IgG Purification
DEAE Blue DEAE Affi-Gel Blue Affinity

Cibacron Blue

F3GA and DEAE

0.2–1.0 ml serum

7324632 7324634
Antibody Purification
Protein A UNOsphere SUPrA Affinity Recombinant protein A 30 mg/ml human IgG 7324200, 1 x 1 ml 7324201, 5 x 1 ml 7324202, 1 x 5 ml
Protein A Affi-Prep Protein A Affinity Protein A 8–10 mg mouse
monoclonal lgG1;
16–23 mg human lgG
7324600 7324602
CHT Type I CHT Ceramic
Hydroxyapatite Type I, 40 µm
Hydroxyapatite (Ca5(PO4)3OH)2 25–60 mg human lgG 7324322 7324324
CHT Type II CHT Ceramic
Hydroxyapatite
Type II, 40 µm
Hydroxyapatite (Ca5(PO4)3OH)2 15–25 mg human lgG 7324332 7324334
Affinity-Tagged Protein Purification
IMAC** Nuvia IMAC Affinity Nitrilotriacetic acid ≥40 mg recombinant
histidine-tagged protein
7800811 7800812
IMAC** Profinity IMAC Affinity Iminodiacetic acid ≥15 mg recombinant
Histidine-tagged protein
7324610 7324612 7324614
GST** Profinity GST Affinity Glutathione ≥10 mg/ml recombinant
GST-tagged protein
7324620 7324622 7324624
Purification of Serum Proteins, Enzymes
Blue Affi-Gel Blue Affinity Cibacron Blue
F3GA
0.2 ml serum
11 mg albumin
7324642 7324644

Cartridge by Application Support Type of Separation Chemical Form Protein Capacity/ml* Catalog Numbers by Cartridge Size
1 x 5
ml
5 x 5
ml
5 x 10
ml
1 x 50
ml
5 x 50
ml
Desalting, Buffer Exchange
P-6 Bio-Gel P-6 Gel filtration Polyacrylamide 10–22% CV*** 7324502 7324504 7325304 7325312 7325314

* Protein capacity based on bulk support; check individual instruction manuals for run conditions and specifications.
** IMAC and GST cartridges are designed for use with the Profinia Protein Purification System.
*** Column volume.

PDF Bulletin 5584 Download the Bio-Scale Mini Cartridges Product Information Sheet for more information.

Bio-Scale Mini Profinity IMAC Cartridges are 1 ml and 5 ml Bio-Scale Mini Cartridges used for the purification of recombinant histidine (His)-tagged proteins.


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Description

Bio-Scale Mini Profinity IMAC cartridges are 1 ml and 5 ml Bio-Scale Mini cartridges filled with Profinity Ni-charged IMAC resin. They are used for the purification of recombinant histidine (His)-tagged proteins by immobilized metal affinity chromatography (IMAC).

IMAC is a powerful purification technique that relies on the affinity that His-tag has for immobilized transition metals. Profinity IMAC resins, based on UNOsphere™ beads, contain iminodiacetic acid (IDA) as the chelating ligand for di- or trivalent metal ions. Its chemical structure allows highly selective binding of recombinant His-tagged proteins when charged with Ni2+ or other transition metals, such as Zn2+ or Cu2+. Profinity IMAC offers high capacity at high flow rates. It can be used under either nondenaturing or denaturing conditions.

Features of Profinity IMAC resins include:

  • Optimal ligand density for higher purity of target protein
  • Superb mechanical strength
  • Excellent pressure-flow properties, for high maximum operating pressures and high flow rates, which allow rapid purification as well as rapid column cleaning and reequilibration
  • Stable from pH 1 to 14
  • Compatible with denaturing agents, detergents, and reducing agents

Bio-Scale Mini cartridges have a double-wall cartridge design* that provides extra durability and allows easy, reliable runs at pressures up to 45 psi with the aqueous buffers most commonly used for protein separation. Convenient, disposable, and ready to use, these cartridges are designed for use with BioLogic™ systems, a peristaltic pump, or any chromatography system. Bio-Scale Mini affinity cartridges can also be run on the Profinia™ protein purification system. For simple step elution, the cartridges can be used with a Luer-Lok syringe. Fittings are available for connection to HPLC- and FPLC-type systems.

Other features include:

  • Simple luer fittings for easy connection to any system
  • Chemically compatible polypropylene parts
  • A wide range of chromatography media

More Information

High Performance Columns For Antibody and Tagged Protein PurificationsLearn more about Bio-Rad’s suite of high-performance chromatography columns designed for high-resolution laboratory scale protein purification.







Related Products

Fitting adaptors are available for connection to other low- and medium-pressure chromatography systems:

  • Fittings kit for connection to BioLogic DuoFlow™ systems (732-0113)
  • Fittings kit for connection to older AKTA systems with M6 fittings (732-0111)
  • Fittings kit for connection to NGC™ systems and newer AKTA systems with 1/16th inch/10-32 fittings (732-0112)

Bio-Rad chromatography systems:

 * U.S.patent 7,208,087.


Specifications

Sizes 1 ml and 5 ml bed volumes; desalting cartridges: 10 ml and 50 ml bed volumes
Recommended flow rate Varies for each support type*
Maximum operating pressure 1, 5, and 10 ml: 45 psi
50 ml: 40 psi
Fittings Female luer inlet and male luer outlet
Column material Polypropylene
Frit material Polyethylene (HDPE)
Dimensions 1 ml: 40 mm long x 5.6 mm inner diameter
5 ml: 40 mm long x 12.6 mm inner diameter
10 ml: 80 mm long x 12.6 mm inner diameter
50 ml: 80 mm lomg x 28.2 mm inner diameter
* Recommended flow rates are given for each support type in the instruction manual.  

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Bio-Scale Mini Profinity IMAC Cartridges

7324610
Pkg of 5, 1 ml, prepacked Ni-charged column, 40 mm x 5.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system

This product is being discontinued soon. Please contact customer service for more information.

The following replacement is recommended:

  • EconoFit Profinity IMAC Columns, Ni-charged, 5 x 1 ml (12009298)
Bio-Scale Mini Profinity IMAC Cartridge

7324612
Pkg of 1, 5 ml, prepacked Ni-charged column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system

This product is being discontinued soon. Please contact customer service for more information.

The following replacement is recommended:

  • EconoFit Profinity IMAC Column, Ni-charged, 1 x 5 ml (12009299)
Bio-Scale Mini Profinity IMAC Cartridges

7324614
Pkg of 5, 5 ml, prepacked Ni-charged column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system

This product is being discontinued soon. Please contact customer service for more information.

The following replacement is recommended:

  • EconoFit Profinity IMAC Columns, Ni-charged, 5 x 5 ml (12009300)
1 ml Empty Bio-Scale™ Mini Cartridges

7324660
Pkg of 5, 1 ml, empty chromatography columns, 40 mm x 5.6 mm, max. pressure 45 psi, for use with BioLogic™ systems or any chromatography system

This product is being discontinued soon. Please contact customer service for more information.
5 ml Empty Bio-Scale™ Mini Cartridges

7324661
Pkg of 5, 5 ml, empty chromatography columns, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic™ systems or any chromatography system

This product is being discontinued soon. Please contact customer service for more information.

Documents

Number Description Options
10005455 Instruction Manual, Bio-Scale™ Mini Profinity™ IMAC Cartridges, 1 and 5 ml, Rev D Click to download
6498 Lab-Scale Protein Purification – Column and Cartridge Selection Guide, Rev C Click to download [ Add to Cart (Free) ]
5584 Bio-Scale Mini Cartridges, Product Information Sheet, Rev B Click to download
5514 Automated Purification of a His-Tagged Protein With the Profinia Protein Purification System: Comparison With Another Low-Pressure Chromatography System, Rev A Click to download
5574 Bio-Scale Mini Profinity Cartridges Product Information Sheet, Rev A Click to download
5444 Bio-Scale Mini Profinity IMAC Cartridge Product Information Sheet, Rev A Click to download
5864 Regeneration of Prepacked IMAC Cartridges on the Profinia™ Protein Purification System, Rev A Click to download

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