Bio-Scale™ Mini Profinity eXact™ Cartridges

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Overview

Bio-Scale Mini Profinity eXact Cartridges are 1 ml and 5 ml cartridges of Profinity eXact Resin for on-column purification and cleavage of proteins using the Profinity eXact Fusion-Tag System.
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Description

Description

Bio-Scale Mini Profinity eXact cartridges are 1 ml and 5 ml Bio-Scale Mini cartridges filled with Profinity eXact purification resin. Profinity eXact purification resin consists of a highly engineered subtilisin protease conjugated to an agarose-based matrix. The cartridges are used for on-column purification and cleavage of recombinant proteins using the Profinity eXact fusion-tag system. The novel Profinity eXact fusion-tag system generates a highly purified recombinant protein containing only its native amino acid sequence in a single step, and in a fraction of the time of other methods.

Bio-Scale Mini cartridges have a double-wall cartridge design* that provides extra durability and allows easy, reliable runs at pressures up to 45 psi with the aqueous buffers most commonly used for protein separation. Convenient, ready to use, and disposable, the cartridges have chemically compatible polypropylene parts. Bio-Scale Mini cartridges are designed for use with BioLogic™ systems, a peristaltic pump, or any chromatography system; simple luer fittings make connections easy. For simple step elution, the cartridges can be used with a Luer-Lok syringe. Fittings are available for connection to HPLC- and FPLC-type systems. Bio-Scale Mini Profinity eXact cartridges can also be run on the Profinia protein purification system.

* U.S.patent 7,208,087.

Specifications

Specifications

Sizes
1 ml and 5 ml bed volumes; desalting cartridges: 10 ml and 50 ml bed volumes
Recommended flow rate
Varies for each support type*
Maximum operating pressure
1, 5, and 10 ml: 45 psi
50 ml: 40 psi
Fittings
Female luer inlet and male luer outlet
Column material
Polypropylene
Frit material
Polyethylene (HDPE)
Dimensions
1 ml: 40 mm long x 5.6 mm inner diameter
5 ml: 40 mm long x 12.6 mm inner diameter
10 ml: 80 mm long x 12.6 mm inner diameter
50 ml: 80 mm lomg x 28.2 mm inner diameter
* Recommended flow rates are given for each support type in the instruction manual.
 

Ordering

Ordering

items

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732-4646_tn.jpg
7324646
Pkg of 2, 1 ml, prepacked affinity column, 40 mm x 5.6 mm, max. pressure 45 psi, for use with BioLogic chromatography and Profinia systems
732-4647_tn.jpg
7324647
Pkg of 4, 1 ml, prepacked affinity column, 40 mm x 5.6 mm, max. pressure 45 psi, for use with BioLogic chromatography and Profinia systems
732-4648_tn.jpg
7324648
Pkg of 1, 5 ml, prepacked affinity column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic chromatography and Profinia systems
732-4620_tn.jpg
7324660

Pkg of 5, 1 ml, empty chromatography columns, 40 mm x 5.6 mm, max. pressure 45 psi, for use with BioLogic™ systems or any chromatography system

732-4624_tn.jpg
7324661
Pkg of 5, 5 ml, empty chromatography columns, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic™ systems or any chromatography system

Documents

Documents

Number Description Options
10011164
Bio-Scale Mini Profinity eXact™ Cartridges Manual, Rev B
6484
High-Performance Columns for Antibody and Tagged Protein Purifications Product Information Sheet, Rev B
[ Add to Cart (Free) ]
6498
Lab-Scale Protein Purification – Column and Cartridge Selection Guide, Rev C
[ Add to Cart (Free) ]
5742
Profinity eXact Purification Methods on the Profinia System, Rev. A
5725
Profinia Buffers for Profinity eXact Chromatography Product Information Sheet, Rev A
5766
Purification of Functional Heterotrimeric G-Protein a-Subunits Using the Profinity eXact Fusion-Tag System on the Profinia Protein Purification System, Rev A