Bio-Scale™ Mini Nuvia™ IMAC Cartridges

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Overview

Bio-Scale Mini Nuvia IMAC Cartridges are high-capacity 5 ml cartridges, with rapid mass transfer kinetics, for purification of recombinant histidine-tagged proteins.
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Description

Description

Bio-Scale Mini Nuvia IMAC Cartridges are prepacked with Nuvia Ni2+-charged IMAC Resin for purification of recombinant histidine-tagged proteins.

Nuvia IMAC Resin, based on Nuvia Beads, contains nitrilotriacetic acid (NTA) as the chelating ligand for di- or trivalent metal ions. The chemical structure of NTA permits highly selective binding of recombinant histidine-tagged proteins when charged with Ni2+ or other transition metals, such as Zn2+ or Cu2+.

Double-walled Bio-Scale Mini Cartridges are convenient, ready to use, and disposable. These 5 ml cartridges are designed for use with a peristaltic pump, Biologic™ and NGC™ Chromatography Systems, or any chromatography system.

Features and Benefits of Nuvia IMAC Columns

  • Double-walled cartridge design for extra durability, for pressures up to 45 psi
  • High capacity, even at high flow rates
  • Stable at pH 2–14
  • Chemically compatible polypropylene parts
  • Reusable/disposable format with luer fittings for easy connection to any system

Applications of Nuvia IMAC Columns

For purifying biomolecules in a variety of workflows:

  • Excellent single-step purity via affinity chromatography
  • Quick, efficient separation of biomolecules
  • Contaminant removal
  • Robust base matrix

This IMAC column resin is compatible with 8 M urea, 6 M guanidine HCl, 1 M NaOH, alcohol up to 20% (v/v), and with common detergents, salts, reducing agents, and all commonly used aqueous buffers.

Related Products

Bio-Rad also offers Nuvia™ IMAC Resin for packing into the column of your choice.

Bio-Rad also offers other low-pressure columns for affinity purification.

Fittings are available for connection to HPLC- and FPLC-type systems.

  • Luer to M6 Adaptor Fittings Kit (732-0111)
  • Luer to BioLogic™ and NGC™ Systems Fittings Kit (732-0113)

Bio-Rad chromatography systems:

More Information

Download the Bio-Scale Mini Nuvia IMAC Cartridges Instruction Manual and Quick Start Guide for detailed column packing recommendations and protocols for optimal protein isolation and column regeneration, cleaning, sanitizing, and storage.


Learn more about Bio-Rad’s suite
of high-performance chromatography
columns designed for high-resolution
laboratory scale protein purification.

Specifications

Specifications

Size
5 ml bed volume IMAC cartridges:
Recommended flow rate
7 ml/min
Maximum operating pressure
45 psi
Fittings
Female luer inlet and male luer outlet
Column material
Polypropylene
Frit material
Polyethylene (HDPE)
Dimensions (L x ID)
40 x 12.6 mm

Ordering

Ordering

items

Use the filters below to refine results!

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7800811

Pkg of 1, 5 ml, prepacked Ni-charged column, 40 x 12.6 mm, max. pressure 45 psi, for use with NGC™ or BioLogic™ Systems or any low- or medium-pressure chromatography system

Image
bio-scale-mini-nuvia-imac-nicharged-columns-780-0812-thumb.jpg
7800812
Pkg of 5, 5 ml, prepacked Ni-charged column, 40 x 12.6 mm, max. pressure 45 psi, for use with NGC™ or BioLogic™ Systems or any low- or medium-pressure chromatography system

Accessories

Accessories

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7320113

Low-pressure system fittings kit, includes 1/4–28 female to male and 1/4–28 female to female luers to connect 1 cartridge to BioLogic DuoFlow and NGC Systems

Documents

Documents

Number Description Options
10042131
Instruction Manual, Bio-Scale™ Mini Nuvia™ IMAC Ni-Charged Cartridges, 1 and 5 ml Quick Start Guide, Rev B
10041106
Instruction Manual, Bio-Scale™ Mini Nuvia™ IMAC Ni-Charged Cartridges, 1 and 5 ml, Rev A
10044307
Instruction Manual, Bio-Rad® Nuvia™ IMAC Resin, Rev A
6484
High-Performance Columns for Antibody and Tagged Protein Purifications Product Information Sheet, Rev B
[ Add to Cart (Free) ]
6498
Laboratory-Scale Protein Purification — Column Selection Guide
6725
From Optimization to Automation: Multidimensional (Multi-D) Histidine-Tag Protein Purification, Ver C