Bio-Scale™ Mini Macro-Prep® High Q and Macro-Prep High S Cartridges

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Overview

These columns are 1 ml and 5 ml cartridges filled with Macro-Prep High Q (strong ion exchanger), Macro-Prep High S (strong cation exchanger), or Macro-Prep DEAE support (weak anion exchanger).
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Description

Description

Bio-Scale Mini Macro-Prep High Q, High S, and DEAE cartridges are 1 ml and 5 ml Bio-Scale Mini cartridges filled with either Macro-Prep High Q, Macro-Prep High S, or Macro-Prep DEAE support:

  • High Q — strong anion exchange; chemical form –N+(CH3)3, protein capacity ≥37 mg/ml BSA
  • High S — strong cation exchange; chemical form –SO3-, the ion-moderated partition chromatography technique, protein capacity ≥49 mg/ml human IgG
  • DEAE — weak anion exchange; chemical form –HN+(C2H5)2, protein capacity >35 mg/ml protein

Macro-Prep ion exchange media meet the demands of analytical applications. These rigid, macroporous hydrophilic media provide exceptional capacity, resolution, and throughput in a chemically and mechanically stable form. Macro-Prep media operate well at low and medium pressures and exhibit minimal volume changes in response to variations in pH or ionic strength. These media provide consistent and reproducible results.

Bio-Scale Mini cartridges have a double-wall cartridge design* that provides extra durability and allows easy, reliable runs at pressures up to 45 psi with the aqueous buffers most commonly used for protein separation. Convenient, ready to use, and disposable, the cartridges have chemically compatible polypropylene parts. Bio-Scale Mini cartridges are designed for use with BioLogic systems, a peristaltic pump, or any chromatography system; simple luer fittings make connections easy. For simple step elution, the cartridges can be used with a Luer-Lok syringe. Fittings are available for connection to HPLC- and FPLC-type systems.

* U.S.patent 7,208,087.

Specifications

Specifications

Sizes
1 ml and 5 ml bed volumes; desalting cartridges: 10 ml and 50 ml bed volumes
Recommended flow rate
Varies for each support type*
Maximum operating pressure
1, 5, and 10 ml: 45 psi
Fittings
Female luer inlet and male luer outlet
Column material
Polypropylene
Frit material
Polyethylene (HDPE)
Dimensions
1 ml — 40 mm length x 5.6 mm inner diameter
* Recommended flow rates are given for each support type in the instruction manual.
 

Ordering

Ordering

items

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7324120
Pkg of 5, 1 ml, prepacked ion exchange column, 40 mm x 5.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system
732-4122_tn.jpg
7324122
Pkg of 1, 5 ml, prepacked ion exchange column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system
732-4124_tn.jpg
7324124
Pkg of 5, 5 ml, prepacked ion exchange column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system
732-4130_tn.jpg
7324130
Pkg of 5, 1 ml, prepacked ion exchange column, 40 mm x 5.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system
732-4132_tn.jpg
7324132
Pkg of 1, 5 ml, prepacked ion exchange column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system
732-4134_tn.jpg
7324134
Pkg of 5, 5 ml, prepacked ion exchange column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system
732-4140_tn.jpg
7324140
Pkg of 5, 1 ml, prepacked ion exchange column, 40 mm x 5.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system
732-4142_tn.jpg
7324142
Pkg of 1, 5 ml, prepacked ion exchange column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system
732-4144_tn.jpg
7324144
Pkg of 5, 5 ml, prepacked ion exchange column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system

Documents

Documents

Number Description Options
10006400
Instruction Manual, Bio-Scale™ Mini Macro-Prep® High Q, High S, and DEAE Cartridges, 1 and 5 ml, Rev C
5584
Bio-Scale Mini Cartridges, Product Information Sheet, Rev B
6498
Lab-Scale Protein Purification – Column and Cartridge Selection Guide, Rev C
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6983
Native and Recombinant Protein Purification Resins/Media Selection Card