Bio-Scale™ Mini Macro-Prep® High Q and Macro-Prep High S Cartridges

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Bio-Scale Mini Macro-Prep High Q and Macro-Prep High S Cartridges

Overview

Prepacked Chromatography Cartridge Selection Guide
Cartridge by Application Support Type of Separation Chemical Form Protein Capacity/ml* Catalog Numbers
by Cartridge Size
5 x 1 ml 1 x 5 ml 5 x 5 ml
Protein and Plasmid Purification
High Q Macro-Prep High Q Strong anion exchange –N+(CH3)3 ≥37 mg BSA 7324120 7324122 7324124
UNOsphere Q UNOsphere Q Strong anion exchange –N+(CH3)3 180 mg BSA 7324100 7324102 7324104
UNOsphere S UNOsphere S Strong cation exchange –SO3- 60 mg human lgG 7324110 7324112 7324114
DEAE Macro-Prep DEAE Weak anion exchange –HN+(C2H5)2 >35 mg protein 7324140 7324142 7324144
Protein Purification
High S Macro-Prep High S Strong cation exchange –SO3- ≥49 mg human lgG 7324130 7324132 7324134
IgG Purification
DEAE Blue DEAE Affi-Gel Blue Affinity

Cibacron Blue

F3GA and DEAE

0.2–1.0 ml serum

7324632 7324634
Antibody Purification
Protein A UNOsphere SUPrA Affinity Recombinant protein A 30 mg/ml human IgG 7324200, 1 x 1 ml 7324201, 5 x 1 ml 7324202, 1 x 5 ml
Protein A Affi-Prep Protein A Affinity Protein A 8–10 mg mouse
monoclonal lgG1;
16–23 mg human lgG
7324600 7324602
CHT Type I CHT Ceramic
Hydroxyapatite Type I, 40 µm
Hydroxyapatite (Ca5(PO4)3OH)2 25–60 mg human lgG 7324322 7324324
CHT Type II CHT Ceramic
Hydroxyapatite
Type II, 40 µm
Hydroxyapatite (Ca5(PO4)3OH)2 15–25 mg human lgG 7324332 7324334
Affinity-Tagged Protein Purification
IMAC** Nuvia IMAC Affinity Nitrilotriacetic acid ≥40 mg recombinant
histidine-tagged protein
7800811 7800812
IMAC** Profinity IMAC Affinity Iminodiacetic acid ≥15 mg recombinant
Histidine-tagged protein
7324610 7324612 7324614
GST** Profinity GST Affinity Glutathione ≥10 mg/ml recombinant
GST-tagged protein
7324620 7324622 7324624
Purification of Serum Proteins, Enzymes
Blue Affi-Gel Blue Affinity Cibacron Blue
F3GA
0.2 ml serum
11 mg albumin
7324642 7324644

Cartridge by Application Support Type of Separation Chemical Form Protein Capacity/ml* Catalog Numbers by Cartridge Size
1 x 5
ml
5 x 5
ml
5 x 10
ml
1 x 50
ml
5 x 50
ml
Desalting, Buffer Exchange
P-6 Bio-Gel P-6 Gel filtration Polyacrylamide 10–22% CV*** 7324502 7324504 7325304 7325312 7325314

* Protein capacity based on bulk support; check individual instruction manuals for run conditions and specifications.
** IMAC and GST cartridges are designed for use with the Profinia Protein Purification System.
*** Column volume.

PDF Bulletin 5584 Download the Bio-Scale Mini Cartridges Product Information Sheet for more information.

These columns are 1 ml and 5 ml cartridges filled with Macro-Prep High Q (strong ion exchanger), Macro-Prep High S (strong cation exchanger), or Macro-Prep DEAE support (weak anion exchanger).
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Ordering Information


Description

Bio-Scale Mini Macro-Prep High Q, High S, and DEAE cartridges are 1 ml and 5 ml Bio-Scale Mini cartridges filled with either Macro-Prep High Q, Macro-Prep High S, or Macro-Prep DEAE support:

  • High Q — strong anion exchange; chemical form –N+(CH3)3, protein capacity ≥37 mg/ml BSA
  • High S — strong cation exchange; chemical form –SO3-, the ion-moderated partition chromatography technique, protein capacity ≥49 mg/ml human IgG
  • DEAE — weak anion exchange; chemical form –HN+(C2H5)2, protein capacity >35 mg/ml protein

Macro-Prep ion exchange media meet the demands of analytical applications. These rigid, macroporous hydrophilic media provide exceptional capacity, resolution, and throughput in a chemically and mechanically stable form. Macro-Prep media operate well at low and medium pressures and exhibit minimal volume changes in response to variations in pH or ionic strength. These media provide consistent and reproducible results.

Bio-Scale Mini cartridges have a double-wall cartridge design* that provides extra durability and allows easy, reliable runs at pressures up to 45 psi with the aqueous buffers most commonly used for protein separation. Convenient, ready to use, and disposable, the cartridges have chemically compatible polypropylene parts. Bio-Scale Mini cartridges are designed for use with BioLogic systems, a peristaltic pump, or any chromatography system; simple luer fittings make connections easy. For simple step elution, the cartridges can be used with a Luer-Lok syringe. Fittings are available for connection to HPLC- and FPLC-type systems.

* U.S.patent 7,208,087.


Specifications

Sizes 1 ml and 5 ml bed volumes; desalting cartridges: 10 ml and 50 ml bed volumes
Recommended flow rate Varies for each support type*
Maximum operating pressure 1, 5, and 10 ml: 45 psi
Fittings Female luer inlet and male luer outlet
Column material Polypropylene
Frit material Polyethylene (HDPE)
Dimensions 1 ml — 40 mm length x 5.6 mm inner diameter
* Recommended flow rates are given for each support type in the instruction manual.  

Ordering

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Bio-Scale Mini Macro-Prep High Q Cartridges

7324120
Pkg of 5, 1 ml, prepacked ion exchange column, 40 mm x 5.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system

This product is being discontinued soon. Please contact customer service for more information.

The following replacement is recommended:

  • EconoFit Macro-Prep High Q Columns, 5 x 1 ml (12009267)
Bio-Scale Mini Macro-Prep High Q Cartridge

7324122
Pkg of 1, 5 ml, prepacked ion exchange column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system

This product is being discontinued soon. Please contact customer service for more information.

The following replacement is recommended:

  • EconoFit Macro-Prep High Q Column, 1 x 5 ml (12009268)
Bio-Scale Mini Macro-Prep High Q Cartridges

7324124
Pkg of 5, 5 ml, prepacked ion exchange column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system

This product is being discontinued soon. Please contact customer service for more information.

The following replacement is recommended:

  • EconoFit Macro-Prep High Q Columns, 5 x 5 ml (12009269)
Bio-Scale Mini Macro-Prep High S Cartridges

7324130
Pkg of 5, 1 ml, prepacked ion exchange column, 40 mm x 5.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system

This product is being discontinued soon. Please contact customer service for more information.

The following replacement is recommended:

  • EconoFit Macro-Prep High S Columns, 5 x 1 ml (12009270)
Bio-Scale Mini Macro-Prep High S Cartridge

7324132
Pkg of 1, 5 ml, prepacked ion exchange column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system

This product is being discontinued soon. Please contact customer service for more information.

The following replacement is recommended:

  • EconoFit Macro-Prep High S Column, 1 x 5 ml (12009271)
Bio-Scale Mini Macro-Prep High S Cartridges

7324134
Pkg of 5, 5 ml, prepacked ion exchange column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system

This product is being discontinued soon. Please contact customer service for more information.

The following replacement is recommended:

  • EconoFit Macro-Prep High S Columns, 5 x 5 ml (12009272)
Bio-Scale Mini Macro-Prep DEAE Cartridges

7324140
Pkg of 5, 1 ml, prepacked ion exchange column, 40 mm x 5.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system

This product is being discontinued soon. Please contact customer service for more information.

The following replacement is recommended:

  • EconoFit Macro-Prep DEAE Columns, 5 x 1 ml (12009264)
Bio-Scale Mini Macro-Prep DEAE Cartridge

7324142
Pkg of 1, 5 ml, prepacked ion exchange column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system

This product is being discontinued soon. Please contact customer service for more information.

The following replacement is recommended:

  • EconoFit Macro-Prep DEAE Column, 1 x 5 ml (12009265)
Bio-Scale Mini Macro-Prep DEAE Cartridges

7324144
Pkg of 5, 5 ml, prepacked ion exchange column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system

This product is being discontinued soon. Please contact customer service for more information.

The following replacement is recommended:

  • EconoFit Macro-Prep DEAE Columns, 5 x 5 ml (12009266)

Documents

Number Description Options
10006400 Instruction Manual, Bio-Scale™ Mini Macro-Prep® High Q, High S, and DEAE Cartridges, 1 and 5 ml, Rev C Click to download
5584 Bio-Scale Mini Cartridges, Product Information Sheet, Rev B Click to download
6498 Lab-Scale Protein Purification – Column and Cartridge Selection Guide, Rev C Click to download [ Add to Cart (Free) ]
6983 Native and Recombinant Protein Purification Resins/Media Selection Card Click to download

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