Bio-Scale Mini Affi-Prep Prepacked Protein A Resin Cartridges

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Overview

Bio-Scale Mini Affi-Prep Prepacked Protein A Resin Cartridges are 1 ml and 5 ml cartridges filled with Affi-Prep Protein A Resin, which can be used to yield highly purified immunoglobulins (IgGs)
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Description

Description

Bio-Scale Mini Affi-Prep protein A Resin Cartridges are 1 ml and 5 ml cartridges filled with Affi-Prep Protein A Resin. Affi-Prep Protein A Resin can be used to yield highly purified immunoglobulins (IgGs), selectively remove IgG prior to analysis of other immunoglobulin classes, or adsorb immune complexes for antigen purification. Protein A binds to the Fc region of immunoglobulins, especially IgG from mammalian species. The cartridges can be used for method scouting or simple purifications.

Features and Benefits

  • Binds a wide range of monoclonal and polyclonal antibodies, including mouse IgG1
  • Macroporous polymeric support is not compressible under normal operating conditions
  • Compatible with most commonly used chromatographic buffers
  • Stable pH range from 2–10
  • Multiple chromatographic cycles without loss of capacity
  • High purity of IgGs
  • High affinity for mammalian IgG
  • High capacities for mouse IgG1 as well as other subclasses with MAPS optimized buffer
  • Easy to use

Bio-Scale Mini Cartridges have a double-wall cartridge design* that provides extra durability and allows easy, reliable runs at pressures up to 45 psi with the aqueous buffers most commonly used for protein separation. Convenient, disposable, and ready to use, these cartridges are designed for use with BioLogic Chromatography Systems, a peristaltic pump, or any chromatography system; luer fittings make connection to any system easy. For simple step elution, the cartridges can be used with a standard syringe.

More Information

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7324600
Pkg of 5, 1 ml, prepacked affinity column, 40 mm x 5.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system
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7324602
Pkg of 1, 5 ml, prepacked affinity column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system

Accessories

Accessories

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7320113
Low-pressure system fittings kit, includes 1/4–28 female to male and 1/4–28 female to female luers to connect 1 cartridge to BioLogic DuoFlow™ and NGC Systems
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7320112
Low-pressure adaptor fittings kit, includes luer to 10-32 fittings to connect 1 cartridge to an HPLC system
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7320111
Low-pressure adaptor fittings kit, includes luer to M6 fittings to connect 1 cartridge to an FPLC system

Documents

Documents

Number Description Options
6498
Lab-Scale Protein Purification – Column and Cartridge Selection Guide, Rev C
[ Add to Cart (Free) ]
5584
Bio-Scale Mini Cartridges, Product Information Sheet, Rev B
5326
Chromatography Column Connection Guide, Rev A
5701
Profinia Buffers for Protein A and Protein G Chromatography PIS, Rev B
5726
Profinia Protein A and G methods Product Information Sheet, Rev A
5712
The Profinia Protein Purification System Simplifies Antibody Purification With Protein A, Rev A
LIT230
Instruction Manual, Affi-Prep Protein A Matrix
LIT79
Instruction Manual, Affi-Prep Protein A MAPS II Kit