The KallestadAutoimmune ANCA IFA Complete Kits contain all necessary reagents for performing tests: human neutrophil substrate slides, FITC conjugate, PBS, mounting medium, negative and positive controls, Evans blue counterstain, cover slips and blotters. Kallestadis the most trusted name in autoimmune testing for more than 25 years. Customers can test for p-ANCA and c-ANCA on a single slide with large cells for clear reactivity. All controls are pre-diluted and ready to use. Wells are spaced for use with a multichannel pipette. Kits are packaged for flexible volume ordering and automation options are available.
Human anti-neutrophil cytoplasmic antibodies (ANCA) are directed against antigenic components mainly present in primary granules of neutrophils. They are serological markers of primary necrotizing systemic vasculitis, particularly in granulomatosis with polyangiitis (GPA) formerly known as Wegener’s granulomatosis. In addition, they have a prognostic interest because, in most cases, their titer is usually correlated to clinical activity during the disease. The IFA method is considered the gold standard for detecting ANCA. When fixed in ethanol, two types of ANCA staining reaction patterns occur: cytoplasmic (c-ANCA) and perinuclear (p-ANCA). The p-ANCA reactions can be confirmed by repeat testing on formalin-fixed slides where p-ANCA reactions convert to c-ANCA.
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