Process Chromatography Webinars

Ask an Expert — 15 Minutes

 

Strategies to Address Large Molecule Purification Challenges

This webinar delves into innovative strategies to surmount these challenges, employing design of experiments (DOE) methodologies and offering insightful case studies centered around plasma protein and virus purification. These real-world applications showcase tangible enhancements in productivity and overall process economics within large molecule purification.

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  CHT Chromatography Media: 5 Essential Facts

  For over 30 years, CHT™ Ceramic Hydroxyapatite Chromatography Media has been a trusted, proven purification solution for many biopharmaceutical manufacturing processes. CHT provides exceptional removal of impurities when other chromatography resins fall short to provide the same level of purity.

• AAV Purification Using AEX and Mixed-Mode Chromatography

  This webcast presents a study exploring different chromatography workflow solutions for purifying recombinant adeno-associated viruses (rAAV), a promising vector for gene therapy due to its safety and potential applications.

• mAb Purification Process Development Using Mixed-Mode Resins

  This webcast covers DOE strategies for process development, including screening, modeling, and optimization, and the selection of models and tools for each stage, as well as data evaluation using mixed-mode resins.

• A Polishing Strategy for Removing Impurities in Bispecific Antibody Purification

  A study using asymmetric and symmetric IgG-like bsAb constructs demonstrated an effective polishing strategy using CHT™ Media. The method achieved excellent removal of byproducts and impurities.

• Development of IEX Purification Process for Lentiviral Vectors

  This webcast explores the development of LVV using chromatographic adsorbents, utilizing high-throughput screening to anion exchange resins.

• Purification of DNA Oligonucleotides using Anion Exchange Chromatography

  This webcast presents data on the purification of DNA oligonucleotides using Nuvia HP-Q Resin, a high-performance strong anion exchanger.

• Purification of Biosimilar Insulin from E. coli

  This webcast presents new purification workflow for recombinant human insulin from E. coli using CEX resins.

• CHT Prepacked Process-Scale Columns

  This webcast showcases performance data for GMP ready CHT prepacked process-scale columns, demonstrating excellent column performance and shipping stability

• Alternative to a Three-Step mAb Purification Process

  This webcast compares a three-step mAb purification to a two-step mAb purification workflow using mixed-mode chromatograpy.

• What You Need to Know About Packing Resins at Process Scale

  Dr. Mark A. Snyder, to learn best practices and guidelines for successfully packing different types of chromatography resins.

• Manufacturing a Recombinant Retrovirus — Downstream Purification Process Development

  This webcast details the importance of resin screening, endonuclease treatment, process development, and the scale-up purification of a retrovirus-like particle intended for use in human subjects.

• Mixed-Mode Chromatography — Optimizing Target Purity and Recovery with Buffer Additives

  This webcast showcases the unique selectivity of mixed-mode chromatography and detail the use of buffer additives for optimal target purity and yield.

• Efficient and Rapid Purification of E. coli Expressed Toxin Recombinant Protein Fragments

  Dr. Lees presents the purification process for rTTHc using polymeric chromatography resins. rTTHc was expressed at high levels as a soluble, properly folded intracellular protein in Fina Bio proprietary Escherichia coli strain.

• Assessing Viral Clearance in Early Phase Process Development

  Viral clearance studies are part of a multifaceted approach to ensure the safety of biopharmaceutical products. In order to prevent costly changes to a manufacturing process, it is important to assess each operation unit for its efficiency on the removal or inactivation of adventitious agents early on during downstream process development.

• Mixed-Mode Chromatography Resins for Biomolecule Purification

  This webinar examines mixed-mode chromatography for biomolecule purification, which has emerged as a viable purification method for biomolecules that are otherwise difficult to purify using traditional platforms and other established means.

• Virus Purification Strategies and Considerations

  Alternative strategies are needed for achieving efficient virus purification. This webinar will provide a snapshot of the resins considered, and the reasons for their selection, in order to achieve the required yield and purity.

• AEX Mixed-Mode Chromatography Resin for High Selectivity and Recovery

  This webinar introduces the features of Nuvia aPrime 4A Resin, for high-purity and recovery over traditional chromatography resins.

• CHT Column Packing is Easier Than You Think

  This webinar presents best practices and guidelines for packing CHT Ceramic Hydroxyapatite Media at a process scale.

• Nuvia HP-Q — A New High Performance AEX Resin

  In this webinar, Dr. Jiali Liao presents case studies and design features to meet manufacturing demands.