Western Blot University

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Western Blot University

Western Blot University: Principles, Techniques, and Resources

These self paced five part online courses are for anyone who has ever asked how to perform a western blot and would like to have a detailed understanding of western blotting principles at each step of the workflow.

Western blotting is a powerful technique that allows you to positively detect your proteins, estimate quantities, and determine their molecular weights starting from a mixture of proteins extracted from cells or tissues.

A certificate of completion will be awarded to those who complete each class.

Register for Online Courses

Western Blot Course Topics and Instructors

Classes are taught by Bio Rad's Field Application Specialists who have diligently compiled all the questions they have received from scientists and have designed these courses to be both comprehensive and efficient. Each class will include approximately 45 min of video instruction, quizzes and links to valuable western blotting resources.

When you sign up for a class, you will automatically have access to private topic specific, online communities. These forums are a place to ask questions, see answers, and share ideas with Bio Rad scientists and your peers.

Title and Topics Instructor  
Fundamentals of Western Blotting Course
#1: Sample Preparation
  • How western blots are used in biological research
  • Experimental design considerations before you begin your project
  • Reagents and methods used for good sample preparation
Headshot of Paul Pease, PhD Paul Pease, PhD,
Proteomics Field Application Specialist
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Fundamentals of Western Blotting Course
#2: Gel Electrophoresis and Transfer
  • Principles of gel electrophoresis
  • Selecting the best gel for your application
  • Select and optimize the best transfer technique for your application
Headshot of Gary F. Ross, PhD Gary F. Ross, PhD,
Proteomics Field Application Specialist
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Fundamentals of Western Blotting Course
#3: Immunodetection
  • How antibodies are used to detect target proteins
  • Choosing the right blocking agent
  • How to select and optimize antibodies
Headshot of Mauhamad Baarine, PhD Mauhamad Baarine, PhD,
Field Application Specialist
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Fundamentals of Western Blotting Course
#4: Image Acquisition
  • Chemiluminescence and fluorescence detection in western blots
  • Optimize your experiment to get the best possible sensitivity
  • Overview of imaging technologies
Headshot of Brad VanderWielen, PhD Brad VanderWielen, PhD,
Field Application Specialist
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Fundamentals of Western Blotting Course
#5: Image Analysis
  • Removing background from your data
  • How to quantify your target protein
  • Normalizing your data to controls
  • Calculating relative expression levels of your target protein
Headshot of Kenneth Oh, PhD Kenneth Oh, PhD,
Collaborations, Applications, and New Technology Senior Global Manager
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