Ultra-Sensitive Quantification of Genome Editing Events by Droplet Digital PCR (ddPCR)

While powerful, the efficiency of gene editing is cell type-dependent and often low (less than 5%), particularly in primary cells or iPSCs. Here we introduce Droplet Digital PCR Assay strategies sensitive enough to detect edited alleles present at a frequency less than 0.5%. Learn how these methods are useful for ultra-sensitive detection of edited alleles and offer a rapid, low-cost readout for the technical optimization of genome editing protocols.