Learn Why You Might Be One of the 90% Using Unsuitable qPCR Reference Genes

Learn Why You Might Be One of the 90 percent Using Unsuitable qPCR Reference Genes

March 3, 2015
Erik Wilson, PhD and
Tom Fletcher, PhD
Technical Support Consultants
Bio-Rad Life Science Research

14.57 min

Erik Wilson and Tom Fletcher of Bio-Rad teach you why to use, how to select, and how to evaluate suitable reference genes.

Quantitative PCR (qPCR) is an excellent method for measuring gene expression but relies on the use of a suitable uniformly expressed reference gene or genes to control for the amount of RNA in each sample. Many researchers select commonly used reference genes that may not be suitable for their system. It is necessary to test whether differences in organisms, conditions, or treatments affect the uniform expression of potential reference genes. We examine the use of Bio-Rad’s PrimePCR Reference Gene Plates to test and evaluate reference genes.