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Next-generation sequencing (NGS) platforms are currently being used to quantify rare species targets in samples. Heavy processing of the starting template in the NGS experimental workflow can result in biases in representing these targets. In this webinar, we discuss Droplet Digital PCR (ddPCR) applications for cross-validating NGS data for rare species targets and the excellent capability of ddPCR quantification of NGS libraries to optimize sequencing performance.