Utilizing A User-Friendly Methylation-Sensitive Restriction Enzyme (MSRE) Protocol And Examining Results
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In this white paper we review MSRE-based methylation detection and explain critical parameters for optimal assay design to achieve unprecedented sensitivity, linearity, and robustness when used with ddPCR. Advantages of this method include negligible DNA degradation and a minimum amount of required starting material. These characteristics make it well suited for applications that require liquid biopsy samples. Additionally, because the DNA is preserved, other genetic alterations such as copy number variants and single nucleotide variants can be performed on the same sample.
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